Browsing by Subject "Imaging"
Now showing 1 - 19 of 19
Results Per Page
Sort Options
Item A 20-coil array system for high-throughput dynamic contrast-enhanced mouse MRI(2011-12) Ramirez, Marc Stephen; Bankson, James A.; Hazle, John D.MRI is a versatile tool for systematically assessing anatomical and functional changes in small animal models of human disease. Its noninvasive nature makes MRI an ideal candidate for longitudinal evaluation of disease progression in mice; however achieving the desired level of statistical power can be expensive in terms of imaging time. This is particularly true for cancer studies, where dynamic contrast-enhanced (DCE-) MRI, which involves the repeated acquisition of anatomical images before, during, and after the injection of a paramagnetic contrast agent, is used to monitor changes in tumor vasculature. A means of reducing the overall time required to scan multiple cohorts of animals in distinct experimental groups is therefore highly desirable. Multiple-mouse MRI, in which several animals are simultaneously scanned in a common MRI system, has been successfully used to improve study throughput. However, to best utilize the next generation of small-animal MRI systems that will be equipped with an increased number of receive channels, a paradigm shift from simultaneously scanning as many animals as possible to scanning a more manageable number, at a faster rate, must be considered. Given a small-animal MRI system with 16 available receive channels, the simulations described in this work explore the tradeoffs between the number of animals scanned at once and the number of array elements dedicated to each animal for maximizing throughput. An array system consisting of 15 receive and 5 transmit coils allows throughput-optimized acceleration of a DCE-MRI protocol by a combination of multi-animal and parallel imaging techniques. The array system was designed and fabricated for use on a 7.0-T / 30-cm MRI system, and tested for high-throughput imaging performance in phantoms. Results indicate that up to a nine-fold throughput improvement is possible without sacrificing image quality compared to standard single-animal imaging hardware. A DCE-MRI study throughput improvement of just over six times that achieved with conventional single-mouse imaging was realized. This system will lower the barriers for DCE-MRI in preclinical research and enable more thorough sampling of disease pathologies that progress rapidly over time.Item Atomic Force Microscope Based Near-field Imaging for Probing Cell Surface Interactions(2013-03-26) Amini, SinaNear-membrane and trans-membrane proteins and their interactions with the extracellular matrix (ECM) can yield valuable information about cell dynamics. However, advances in the field of nanoscale cellular processes have been hindered, in part, due to limits imposed by current technology. In this work, a novel evanescent field (EF) imaging technique is designed, modeled, created and tested for near-field imaging in the apical surface of cells. This technique and F?rster resonance energy transfer (FRET) were used to investigate interactions between integrins on the cell surface and the ECM protein, fibronectin. The goal was to monitor changes in the integrin density at the cell surface as a function of clustering after binding to fibronectin on the microsphere surface. For the EF technique, quantum dot (QD)-embedded polystyrene microspheres were used to couple light into whispering gallery modes (WGMs) inside the microspheres; the resulting EF at the surface of the microsphere was used as a near-field excitation source with ~50 nm axial resolution for exciting fluorescently-labeled integrins. For FRET measurements (~10 nm axial resolution), QDs (donors) were coated on the surface of microspheres and energy transfer to red fluorescent protein (RFP)-integrin constructs (acceptors) studied. In both techniques, the QD-modified microspheres were mounted on atomic force microscope (AFM) cantilevers, functionalized with fibronectin, and brought into contact with fluorescently-labeled HeLa or vascular smooth muscle (VSM) cells. The results obtained from both methods show the clustering and activity of the integrins and are in good agreement with each other. Amsterdam discrete dipole approximation (ADDA) was used to study the effects of inhomogeneous surrounding refractive index on the quality factor and position of the WGMs due to the attachment of a microsphere to an AFM cantilever. WGMs of various QD-embedded microspheres mounted on AFM cantilevers were experimentally measured and shown to be consistent with the model.Item Characterization of aggregate shape properties using a computer automated system(Texas A&M University, 2005-02-17) Al Rousan, Taleb MustafaShape, texture, and angularity are among the properties of aggregates that have a significant effect on the performance of hot-mix asphalt, hydraulic cement concrete, and unbound base and subbase layers. Consequently, there is a need to develop methods that can quantify aggregate shape properties rapidly and accurately. In this study, an improved version of the Aggregate Imaging System (AIMS) was developed to measure the shape characteristics of both fine and coarse aggregates. Improvements were made in the design of the hardware and software components of AIMS to enhance its operational characteristics, reduce human errors, and enhance the automation of test procedure. AIMS was compared against other test methods that have been used for measuring aggregate shape characteristics. The comparison was conducted based on statistical analysis of the accuracy, repeatability, reproducibility, cost, and operational characteristics (e.g. ease of use and interpretation of the results) of these tests. Aggregates that represent a wide range of geographic locations, rock type, and shape characteristics were used in this evaluation. The comparative analysis among the different test methods was conducted using the Analytical Hierarchy Process (AHP). AHP is a process of developing a numerical score to rank test methods based on how each method meets certain criteria of desirable characteristics. The outcomes of the AHP analysis clearly demonstrated the advantages of AIMS over other test methods as a unified system for measuring the shape characteristics of both fine and coarse aggregates. A new aggregate classification methodology based on the distribution of their shape characteristics was developed in this study. This methodology offers several advantages over current methods used in practice. It is based on the distribution of shape characteristics rather than average indices of these characteristics. The coarse aggregate form is determined based on three-dimensional analysis of particles. The fundamental gradient and wavelet methods are used to quantify angularity and surface texture, respectively. The classification methodology can be used for the development of aggregate shape specifications.Item Development of Advanced Optics and High Resolution Instrumentation for Mass Spectrometry Based Proteomics(2010-01-14) Sherrod, Stacy D.Imaging mass spectrometry (MS) analysis allows scientists the ability to obtain spatial and chemical information of analytes on a wide variety of surfaces. The ability to image biological analytes is an important tool in many areas of life science research, including: the ability to map pharmaceutical drugs in targeted tissue, to spatially determine the expression profile of specific proteins in healthy vs. diseased tissue states, and to rapidly interrogate biomolecular microarrays. However, there are several avenues for improving the imaging MS experiment for biological samples. Three significant directions this work addresses include: (1) reducing chemical noise and increasing analyte identification by developing sample preparation methodologies, (2) improving the analytical figures of merit (i.e., spatial resolution, analysis time) by implementing a spatially dynamic optical system, and (3) increasing both mass spectral resolution and ion detection sensitivity by modifying a commercial time-of-flight (TOF) MS. Firstly, sample methodology schemes presented in these studies consist of obtaining both ?top-down? and ?bottom-up? information. In that, both intact mass and peptide mass fingerprinting data can be obtained to increase protein identification. This sample methodology was optimized on protein microarrays in preparation for bio tissue analysis. Other work consists of optimizing novel sample preparation strategies for hydrated solid-supported lipid bilayer studies. Sample methods incorporating nanomaterials for laser desorption/ionization illustrate the ability to perform selective ionization of specific analytes. Specifically, our results suggest that silver nanoparticles facilitate the selective ionization of olefin containing species (e.g., steroids, vitamins). Secondly, an advanced optical design incorporating a spatially dynamic optical scheme allows for laser beam expansion, homogenization, collimation, shaping, and imaging. This spatially dynamic optical system allows user defined beam shapes, decreases analysis times associated with mechanical movement of the sample stage, and is capable of increasing the MS limits of detection by simultaneously irradiating multiple spots. Lastly, new data acquisition strategies (multiple anode detection schemes) were incorporated into a commercial time-of-flight mass spectrometer to increase both sensitivity and resolution in a matrix assisted laser desorption/ionization mass spectrometer. The utility of this technique can be applied to many different samples, where high mass spectral resolution allows for increased mass measurement accuracy.Item Development of an electronically tunable ultra-wideband radar imaging sensor and its components(Texas A&M University, 2006-08-16) Han, JeongwooNovel microwave transmitter and receiver circuits have been developed for implementing UWB (Ultra-Wideband) impulse radar imaging sensor operating in frequency band 0.2 to 4 GHz. with tunable operating frequency band. The fundamental system design parameters such as the required transmitting pulse power and the pulse duration were estimated for a presumed specific application, the pavement assessment. The designed transmitter is the tunable monocycle pulse generator with tuning capability for the output pulse duration from 450- to 1200- ps, and has relatively high transmitting pulse power from 200 to 400 mW. Tuning of the pulse duration was implemented by novel PIN diode switch configuration and decoupling circuit, and boosting of transmitting pulse power was made possible by using a high power pulse driving circuit and SRD coupling circuit. The synchronous sampling receiver system was designed by using the integrated sampling mixer and two reference clock oscillators placed in the transmitter and receiver respectively for timing control. A novel integrated CSH (Coupled-Slotline Hybrid)sampling mixer has been developed along with the design of the strobe pulse generator appropriate for the impulse radar system. The integrated sampling mixer has unprecedented conversion loss of 2.5 dB for the pulse signal, bandwidth 5.5 GHz, and dynamic range 50 dB. The introduced UWB LNA (Low Noise Amplifier) design operating up to 4 GHz should be useful for weak signal detection applications. The design of the UWB microstrip quasi-horn antenna was optimized for short pulse transmission with respect to the input return loss and the pulse stretching effect. For signal detection in the signal processing stage, the background subtraction technique and B-scan data format were used. A novel signal monitoring technique was introduced in the signal processing to compensate the frequency modulation effect of the reference clock. The test results for the complete system with respect to some sample multi-layer structures shows good receiving pulse waveform with low distortion, enough pulse penetration depth for 13?? pavement sample structure, and minimum 1-in of range resolution.Item Engineering functionalized gold nanoparticles as a molecular-specific contrast agent to enhance optoacoustic detection of breast cancer cells(2008-12-02) Mohammad Eghtedari; Massoud Motamedi; Vicente Resto; Nicholas A Kotov; Malcolm Brodwick; Gracie VargasMolecular targeting of malignant tumors is a promising field of research that could potentially revolutionize the diagnosis and treatment of many types of cancer including breast. Delivering molecular specific contrast agents to breast cancer cells would enhance the sensitivity and specificity of imaging methods to detect cancer foci at earlier stages, when complete cure is possible. Optoacoustic tomography (OAT) is a non-invasive imaging modality that can be used to produce an image of the distribution of light absorbing components deep within a turbid medium such as human breast. OAT could potentially be used to image breast tumors based on their enhanced angiogenesis; however, its sensitivity and specificity would be limited due to the lack of abnormal angiogenesis at the early stages of tumor growth.\r\nGold nanoparticles generate strong acoustic signal upon pulse laser irradiation and thus are detectable at low concentrations using optoacoustic technique. The goal of this dissertation is to engineer functionalized gold nanoparticles and employ them as a contrast agent for optoacoustic detection of cancer cells.\r\nTo achieve this goal: 1) gold nanoparticles were fabricated in different shape and sizes and their physicochemical properties were optimized for both tumor targeting and optoacoustic detection; 2) the biological properties of fabricated gold nanoparticles were evaluated in vitro and in vivo by determining their stability, toxicity, biodistribution, and molecular targeting properties; 3) the performance of gold nanoparticles to target cancer cells and function as a contrast agent for OAT were assessed in vitro using breast phantoms and then in vivo using animal models.\r\nItem Experimental Evaluation of New Generation Aggregate Image Measurement System(2010-07-14) Gates, Leslie L.The performance of hot mix asphalt, Portland cement concrete, unbound base, and subbase layers in a pavement are significantly affected by aggregate shape characteristics. Classification of coarse and fine aggregate shape properties such as shape (form), angularity, and texture, are important in predicting the performance of pavements. Consequently, there is a need to implement a system that can characterize aggregates without the limitations of the current aggregate classification standards. The Aggregate Image Measurement System (AIMS) was developed as a comprehensive and capable means of measuring aggregate shape properties. A new design of AIMS will be introduced with several modifications to improve the operational and physical components. The sensitivity, repeatability, and reproducibility are analyzed to evaluate the quality of AIMS measurements. The sensitivity of AIMS is evaluated and found to be good for several operational and aggregate parameters. Important operational and environmental factors that could affect the AIMS results are identified and appropriate limits are recommended. AIMS is able to control normal variations in the system without affecting the results. A comprehensive analysis is conducted to determine the repeatability and reproducibility of AIMS for multiple users and laboratories. Single-operator and multi-laboratory precision statements are developed for the test method in order to be implemented into test standards.Item Imaging Heterogeneous Objects Using Transport Theory and Newton's Method(2012-02-14) Fredette, NathanielThis thesis explores the inverse problem of optical tomography applied to two-dimensional heterogeneous domains. The neutral particle transport equation was used as the forward model to simulate how neutral particles stream through and interact within these heterogeneous domains. A constrained optimization technique that uses Newton's method served as the basis of the inverse problem. The capabilities and limitations of the presented method were explored through various two-dimensional domains. The major factors that influenced the ability of the optimization method to reconstruct the cross sections of these domains included the locations of the sources used to illuminate the domains, the number of separate experiments used in the reconstruction, the locations where measurements were collected, the optical thickness of the domain, the amount of signal noise and signal bias applied to the measurements, and the initial guess for the cross section distribution. All of these factors were explored for problems with and without scattering. Increasing the number of sources, measurements and experiments used in the reconstruction generally produced more successful reconstructions with less error. Using more sources, experiments and measurements also allowed for optically thicker domains to be reconstructed. The maximum optical thickness that could be reconstructed with this method was ten mean free paths for pure absorber domains and two mean free paths for domains with scattering. Applying signal noise and signal bias to the measured fluxes produced more error in the reconstructed image. Generally, Newton's method was more successful at reconstructing domains from an initial guess for the cross sections that was greater in magnitude than their true values than from an initial guess that was lower in magnitude.Item Methods and simulations of muon tomography and reconstruction(2016-05) Schreiner III, Henry Fredrick; Schwitters, Roy F.; Dicus, Duane; Lang, Karol; Onyisi, Peter; Navratil, PaulThis dissertation investigates imaging with cosmic ray muons using scintillator-based portable particle detectors, and covers a variety of the elements required for the detectors to operate and take data, from the detector internal communications and software algorithms to a measurement to allow accurate predictions of the attenuation of physical targets. A discussion of the tracking process for the three layer helical design developed at UT Austin is presented, with details of the data acquisition system, and the highly efficient data format. Upgrades to this system provide a stable system for taking images in harsh or inaccessible environments, such as in a remote jungle in Belize. A Geant4 Monte Carlo simulation was used to develop our understanding of the efficiency of the system, as well as to make predictions for a variety of different targets. The projection process is discussed, with a high-speed algorithm for sweeping a plane through data in near real time, to be used in applications requiring a search through space for target discovery. Several other projections and a foundation of high fidelity 3D reconstructions are covered. A variable binning scheme for rapidly varying statistics over portions of an image plane is also presented and used. A discrepancy in our predictions and the observed attenuation through smaller targets is shown, and it is resolved with a new measurement of low energy spectrum, using a specially designed enclosure to make a series of measurements underwater. This provides a better basis for understanding the images of small amounts of materials, such as for thin cover materials.Item Optical coherence tomography for retinal diagnostics(2013-08) Yin, Biwei; Milner, Thomas E.; Rylander, H. Grady (Henry Grady), 1948-Optical Coherence Tomography (OCT) is a non-invasive three-dimensional imaging technique. OCT synthesizes a cross-sectional image from a series of lateral adjacent depth scans, and with a two-dimensional scanning scheme, three-dimensional intensity image of sample can be constructed. Due to its non-invasive capability, OCT has been widely applied in ophthalmology, cardiology and dermatology; and in addition to three-dimensional intensity image construction, various functional OCT imaging techniques have been developed for clinical application. My research is focused on developing functional OCT systems for application in ophthalmology, including polarization-sensitive optical coherence tomography (PS-OCT) for retinal nerve fiber layer (RNFL) birefringence measurement and dual-wavelength photothermal optical coherence tomography (DWP-OCT) for microvasculature blood oxygen saturation (SO2) measurement. In the study, a single-mode-fiber based polarization-sensitive swept-source OCT (PS-SS-OCT) with polarization modulator, polarization-sensitive bulk-optics balanced detection module is constructed and polarization processing methods based on Stokes vectors are applied to determine birefringence. PS-OCT is able to provide human subject's RNFL thickness, phase retardation, and birefringence information. Degradation in the degree of polarization (DOP) along depth is investigated and its difference between four quadrants of RNFL (superior, temporal, inferior and nasal) indicates the structural property difference. DWP-OCT is a novel functional OCT system consisting of a phase-sensitive optical coherence tomography system (PhS-OCT) and two photothermal excitation lasers. PhS-OCT is based on a swept-source laser operating in the 1060 nm wavelength range; the two photothermal excitation lasers with wavelength 770 nm and 800 nm are intensity modulated at different frequencies. PhS-OCT probe beam and two photothermal excitation beams are combined and incident on the sample, optical pathlength (op) change on the sample introduced by two photothermal excitation beams are measured and used for blood SO2 estimation. A polarization microscope is proposed for future study. The polarization microscope is an imaging technique providing molecular structure and orientation based on probe light's polarization state information. The polarization microscope uses a wavelength tunable light source, and can achieve any incident polarization state by a retarder-rotator combination. Specimen's birefringence can be determined based on the changing of detected light amplitude.Item Optically-triggered nanodroplets for enhanced ultrasound and photoacoustic imaging(2015-05) Hannah, Alexander Steward; Emelianov, Stanislav Y.Medical ultrasound imaging is ubiquitous in clinics due to its safety, low cost, portability, and imaging depth. The development of technologies to assist ultrasound in the diagnosis of diseases thus have a potentially broad clinical impact. More recently, photoacoustics has emerged as a complementary, high contrast modality for imaging optical absorption. Injectable dyes and nanoparticles locally amplify ultrasound and photoacoustic signal, helping to identify disease markers and track its progression. We have constructed a dual ultrasound and photoacoustic contrast agent that can be activated using an external optical trigger. In response to pulsed laser irradiation, the particle undergoes a liquid to gas phase change, or vaporization, which emits a strong acoustic wave and results in an echogenic microbubble, simultaneously enhancing contrast for both modalities. We designed and developed several iterations of particles, altering parameters to optimize biocompatibility, cost, and image contrast enhancement, and we then characterized key traits of the particles. Next, we imaged the contrast agents in phantom, ex vivo, and in vivo models to validate the image enhancement, developing image process algorithms to maximize image quality. These optically triggered contrast agents are a valuable tool for minimally invasive, highly specific, early identification of cancer.Item Optimal Maneuvers for Distributed Aperture Imaging Systems(2012-10-19) Fitch, DanielleInterest in space-borne, distributed multi-aperture interferometric systems is driven by a need for continuously sustained imaging with high resolution. Amplitude interferometry systems measure the Fourier components of the image corresponding to the wave vectors (locations in the so-called u-v plane) that are proportional to the relative positions of the apertures. Imaging to specified resolution demands measurement of the Fourier components with adequate signal-to-noise ratio over the interior of a disk in the u-v plane (the resolution disk). In this paper we concentrate on the case in which interferometric measurements are made while the apertures are changing their relative positions. This work discusses heuristic maneuvers and strategies for a system of two space-borne telescopes to cover the frequency plane while optimizing a cost function that includes both a measure of image quality and propulsive effort. The current study is motivated by previous research in which the optimization problem was formulated and the first-order necessary conditions (FONC) derived. The earlier work obtained short time horizon solutions to the FONC for various simple situations, but the complexity of the integro-differential equations for optimal maneuvering have heretofore prevented solution for an optimal maneuver for the entirety of the imaging process. In place of a direct attack on the FONC, the present work investigates various heuristic approaches to minimizing the cost function in the discretized state and discretized time domains in a hexagonal coordinate system. Using three classes of coverage rules, experimentation with a variety of maneuver strategies involving two apertures has led to a number of time-optimal or fuel-optimal solutions based on the initial conditions of the spacecraft. This thesis shows that an optimal maneuver can be determined from the starting positions of the spacecraft and that a self-spiral class of motion seems to be the most beneficial for long term strategies. Future work may focus on strategies for interferometric systems with more than two apertures and with a finer mesh of the hexagonal coordinate system.Item Phase Retrieval with Application to Intensity Correlation Interferometers(2012-10-24) Trahan, Russell 1987-As astronomers and astrophysicists seek to view ever-increasingly distant celestial objects, the desired angular resolution of telescopes is constantly being increased. Classical optics, however, has shown a proportional relationship between the size of an optical telescope and the possible angular resolution. Experience has also shown that prohibitive cost accompanies large optical systems. With these limitations on classical optical systems and with the drastic increase in computational power over the past decade, intensity correlation interferometry (ICI) has seen renewed interest since the 1950?s and 60?s when it was initially conceived by Hanbury Brown and Twiss. Intensity correlation interferometry has the advantage of less stringent equipment precision and less equipment cost when compared to most other forms of interferometry. ICI is thus attractive as a solution to the desire for high angular resolution imaging especially in space based imaging systems. Optical interferometry works by gathering information about the Fourier transform of the geometry of an optical source. An ICI system, however, can only detect the magnitude of the Fourier components. The phase of the Fourier components must be recovered through some computational means and typically some a priori knowledge of the optical source. This thesis gives the physics and mathematical basis of the intensity correlation interferometer. Since the ICI system cannot detect the phase of an optical source's Fourier transform, some known methods for recovering the phase information are discussed. The primary method of interest here is the error-reduction algorithm by Gerchberg-Saxton which was adapted by Fienup to phase retrieval. This algorithm works by using known qualities of the image as constraints; however, sometimes it can be difficult to know what these constraints are supposed to be. A method of adaptively discovering these constraints is presented, and its performance is evaluated in the presence of noise. Additionally, an algorithm is presented to adapt to the presence of noise in the Fourier modulus data. Finally, the effects of the initial condition of the error-reduction algorithm are shown and a method of mitigating its effect by averaging several independent solutions together is shown.Item Photoacoustic image guidance and tissue characterization in cardiovascular applications(2016-12) Dana, Nicholas Pacheco; Suggs, Laura J.; Emelianov, Stanislav Y.; Dunn, Andrew; Tunnell, James; Bouchard, RichardCollectively, cardiovascular diseases continue to be the leading cause of death, across nations and across decades. Improved diagnostic imaging methods offer promise to alleviate the morbidity associated with these diseases. Photoacoustic (PA) imaging is one such method, poised to make a significant impact on cardiovascular imaging, both as a research tool, as well as a clinical imaging modality. Offering the potential of molecular imaging in real-time, PA methods have been demonstrated in proof-of-concept studies tracking myocyte calcium dynamics. These results open the door to non-invasive longitudinal assessment of cardiac electrophysiological function, with implications for drug and contrast agent development. PA image guidance has also been extended to the characterization of cardiac radiofrequency ablation lesions. This method has been demonstrated to utilize endogenous chromophore changes resulting from ablation for the generation of depth-resolved tissue characterization maps, capable of assessing lesion extent. The technique has been subsequently validated by assessing high-intensity focused ultrasound ablation lesions in myocardium, with the hope for offering thermographic capabilities as well. While PA imaging offers such promise in cardiac ablation procedures, it is also in the process of clinical translation for image guidance and characterization in coronary artery disease applications. Research has shown, using Monte Carlo optical modeling, that using a simple dual-wavelength PA imaging technique has great potential for successful visualization of atherosclerotic plaques across multiple tissue types and at clinically relevant multiple millimeters of depth. Collectively these results offer a suite of PA imaging tools with the potential for molecular and thermographic imaging across a broad range of cardiovascular applications.Item Photoacoustic imaging for temperature monitoring and photothermal therapy guidance(2015-08) Dumani Jarquin, Diego Sayed; Emelianov, Stanislav Y.; Aglyamov, Salavat; Cook, JasonCancer is among the leading causes of death in the United States and the world. Using combined ultrasound (US) and photoacoustic (PA) imaging can provide both anatomical and molecular information of cancerous cells for both diagnostic and therapeutic purposes. This thesis is focused on combining these two modalities, in conjunction with nanoparticles, to treat cancerous tumors and monitor the progress of the therapy. Specifically, the work evaluates the use of photoacoustic imaging as a temperature monitoring tool during photothermal therapy. In this study, the mathematical relationship between photoacoustic signal and temperature is presented and validated with a glass tube phantom using gold nanoparticles. Then in vitro and ex vivo studies were performed to validate the effect of photothermal therapy and the monitoring capability of photoacoustic imaging. The results show that photoacoustic imaging can be used to monitor temperature during photothermal therapy. Future directions include in vivo studies of image guided photothermal therapy and survival studies.Item Seismic diffraction imaging methods and applications(2014-05) Decker, Luke Adam; Fomel, Sergey B.; Ren, Kui; Wilson, ClarkSeismic waves can either be reflected or diffracted by subsurface objects depending on the object's geometry. Diffactions can be used to determine details about the small-scale features that generate them, such as karsts, voids, pinchouts, faults, fractures, and salt flanks. Diffraction imaging can have resolution below the typical seismic wavelength. Scattered waves are recorded as significantly lower-energy signal than reflected waves, requiring that diffractions be separated from reflections. I describe three methods of such separation: data-domain plane-wave destruction, Fresnel zone Elimination, and partial-image plane-wave destruction. Once separated, diffractions can be migrated to create a seismic diffraction image and used in velocity analysis. Common-reflection angle migrated diffractions appear flat in dip angle gathers when migrated with correct velocity. I illustrate how this property can be used to determine migration velocity through a process of oriented velocity continuation (OVC). In OVC framework diffraction data are decomposed by slope and migrated over a range of velocities. Velocities corresponding to the flattest slope gathers are picked using semblance as a measure of flatness. This provides an estimate of migration velocity. Stacking gathers corresponding to this chosen velocity generates a seismic diffraction image. Seismic diffraction images provide interpreters with information about small-scale geologic objects that may not be available in conventional images. Scattering features that are interesting for exploration, like voids, caves, fractures, and faults, cause diffractions and can be resolved with better focus in diffraction images than in conventional ones. This is particularly useful with geologically complex carbonate systems. Carbonates are strongly heterogeneous, making them difficult to image with conventional methods. Reservoir porosity is often contained within caves, or small vugs. These features are difficult to characterize with conventional methods because cave reflections have large geometric uncertainties in cave size and location. Velocity analysis of seismic reflection data in carbonates may not highlight vugular porosity particularity well. I illustrate how diffraction images provide improved characterization by highlighting the edges of caves, thus constraining cave geometry, and highlighting more heterogenous zones by measuring the amount of scattering those zones generate.Item Session 1D | Imaging Group Birds-of-a-Feather(2022-05-23) Mazzei, Erin; Willis, Shannon; Jones, Jerrell; McIntosh, Marcia; McKee, MargaretThe TDL Imaging Group was founded in 2019 to address the challenges of cultural heritage imaging among regional practitioners. The groups’ goals include exchanging knowledge on topics such as equipment use, color management, workflows, project management, and other tricks of the trade in a supportive environment. Attendees of this birds-of-a-feather will be able to discuss among peers their imaging specific issues and triumphs, including, but not limited to, how the field has adapted to challenges over the past few years.Item Study of microfluidic measurement techniques using novel optical imaging diagnostics(Texas A&M University, 2007-04-25) Park, JaesungNovel microscale velocity and temperature measurement techniques were studied based on confocal laser scanning microscopy (CLSM) and optical serial sectioning microscopy (OSSM). Two microscopic measurement systems were developed, 1) a CLSM micro particle image velocimetry (PIV) system with a dual Nipkow disk confocal unit (CSU-10), a CW argon-ion laser and an upright microscope, and 2) an OSSM micro- particle tracking velocimetry (PTV) system with an epi-fluorescence microscope and a non-designed specimen to make a three-dimensional (3-D) diffraction particle image. The CLSM micro-PIV system shows a unique optical slicing capability allowing true depth-wise resolved vector field mapping. A comparative study is presented between the CLSM micro-PIV and a conventional epi-fluorescence micro-PIV. Both have been applied to the creeping Poiseuille flows in two different microtubes of 99-????m (Re = 0.00275) and 516-????m ID diameters (Re = 0.021). The CLSM micro-PIV consistently shows significantly improved particle image contrasts, the definition of "optical slicing" and measured flow vector fields more accurately agreeing with predictions based on the Poiseuille flow fields, compared to the conventional micro-PIV. The OSSM micro-PTV technique is applied for a 3-D vector field mapping in a microscopic flow and a Brownian motion tracking of nanoparticles. This technique modifies OSSM system for a micro-fluidic experiment, and the imaging system captures a diffracted particle image having numerous circular fringes instead of an in-focus particle image. The 3-D particle tracking is based on a correlation between the 3-D diffraction pattern of a particle and the defocus distance from a focal plane. A computational program is invented for the OSSM micro-PTV, and provides a 3-D velocity vector field with a spatial resolution of 5.16 ????m. In addition, a concept of nonintrusive thermometry is presented based on the correlation of the Brownian motion of suspended nanoparticles with the surrounding fluid temperature. Detection of fully three-dimensional Brownian motion is possible by the use of the OSSM, and the measured value of mean square displacement (MSD) is compared fairly well with Einstein's predictions.Item Ultrasensitive Magnetometry and Imaging with NV Diamond(2011-08-08) Kim, ChangdongNV centers in a diamond are proving themselves to be good building blocks for quantum information, electron spin resonance (ESR) imaging, and sensor applications. The key feature of the NV is that it has an electron spin that can be polarized and read out at room temperature. The readout is optical, thus the magnetic field imaging can also be done easily. Magnetic field variation with feature sizes below 0.3 microns cannot be directly resolved, and so in this region magnetic resonance imaging must be employed. To realize the full sensitivity of NV diamond, the spin transition linewidth must be as narrow as possible. Additionally, in the case of NV ensembles for micron-sized magnetometers, there must be a high concentration of NV. To this end three techniques are explored: (1) Electron paramagnetic resonance (EPR) imaging with microwave field gradients, (2) Magic angle rotation of magnetic field, and (3) TEM irradiation to optimize the yield of NV in a diamond. For the EPR imaging demonstration a resonant microwave field gradient is used in place of the usual DC magnetic gradient to obtain enough spatial resolution to resolve two very close "double NV" centers in a type Ib bulk diamond. Microfabrication technology enabled the micron-size wire structure to sit directly on the surface of millimeter-scale diamond plate. In contrast to conventional magnetic resonance imaging pulsed ESR was used to measure the Rabi oscillations. From the beating of Rabi oscillations from a "double NV," the pair was resolved using the one-dimension EPR imaging (EPRI) and the spatial distance was obtained. To achieve high sensitivity in nitrogen-doped diamond, the dipole-dipole coupling between the electron spin of the NV center and the substitutional nitrogen (14N) electron must be suppressed because it causes linewidth broadening. Magic angle spinning is an accepted technique to push T2 and T2 * down toward the T1 limit. An experiment was performed using the HPHT diamond with a high concentration of nitrogen, and a rotating field was applied with a microfabricated wire structure to reduce line broadening. In this experiment, ~50% suppression of the linewidth was observed and the effective time constant T2* improved from 114 ns to 227 ns. To achieve the highest possible sensitivity for micro-scale magnetic sensors the concentration of NV should be large. Since the unconverted N are magnetic impurities they shorten T2 and T2*, giving a tradeoff between NV (and therefore N) concentration and sensitivity. To construct a damage monitor, a type Ib HPHT sample was irradiated with electrons from a transmission electron microscope (TEM) and the effects on the ESR transition were seen well before physical damage appeared on the diamond and thus this proved to be a sensitive metric for irradiation damage.