Characterizing the states of yeast cytoplasm using single particle tracking

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dc.contributor.advisorFlorin, Ernst-Ludwig
dc.creatorIbeneche, Chieze Chinenye
dc.date.accessioned2017-03-30T15:58:11Z
dc.date.accessioned2018-01-22T22:31:53Z
dc.date.available2017-03-30T15:58:11Z
dc.date.available2018-01-22T22:31:53Z
dc.date.issued2007-08
dc.description.abstractThe cytoplasmic states of yeast cytoplasm have been characterized using differential interference contrast microscopy and high resolution single particle tracking. Lipid granules naturally occurring in the yeast cytoplasm have been found to diffuse anomalously in the cytoplasm. Two distinct physiological states of yeast cytoplasm were identified with corresponding [Greek small letter alpha] values of [Greek small letter alpha] = 0.66 [plus or minus] 0.1 for the fast moving state and [Greek small letter alpha] = 0.43 [plus or minus] 0.04 for the slow moving state. The generalized diffusion constant D, was identified as a new indicator for cytoplasmic state classification, while a critical value of [Greek small letter alpha] = 0.485 [plus or minus] 0.005 was determined for state transition. Fast moving granules were established as a marker for optimally grown cells, while the origin of the slow moving state was linked to cells constrained to grow under sub-optimal cold conditions.en_US
dc.description.departmentPhysicsen_US
dc.format.mediumelectronicen_US
dc.identifierdoi:10.15781/T27941046
dc.identifier.urihttp://hdl.handle.net/2152/46282
dc.language.isoengen_US
dc.relation.ispartofUT Electronic Theses and Dissertationsen_US
dc.rightsCopyright © is held by the author. Presentation of this material on the Libraries' web site by University Libraries, The University of Texas at Austin was made possible under a limited license grant from the author who has retained all copyrights in the works.en_US
dc.rights.restrictionRestricteden_US
dc.subjectYeast cytoplasmen_US
dc.subjectLipid granulesen_US
dc.subjectSingle particle trackingen_US
dc.titleCharacterizing the states of yeast cytoplasm using single particle trackingen_US
dc.typeThesisen_US
dc.type.genreThesisen_US

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