The Mitotic Spindle Mediates Inheritance of the Golgi Ribbon Structures
| dc.contributor.advisor | Seemann, Joachim | en |
| dc.creator | Wei, Jen-Hsuan | en |
| dc.date.accessioned | 2010-07-12T18:56:39Z | en |
| dc.date.accessioned | 2014-02-19T22:02:31Z | |
| dc.date.available | 2010-07-12T18:56:39Z | en |
| dc.date.available | 2014-02-19T22:02:31Z | |
| dc.date.issued | 2010-05-14 | en |
| dc.description.abstract | The mammalian Golgi ribbon disassembles during mitosis and reforms in both daughter cells after division. Mitotic Golgi membranes concentrate around the spindle poles, suggesting that the spindle may control Golgi partitioning. To test this, cells were induced to divide asymmetrically with the entire spindle segregated into only one daughter cell. A ribbon reforms in the nucleated karyoplasts, whereas the Golgi stacks in the cytoplasts are scattered. However, the scattered Golgi stacks are polarized and transport cargo. Microinjection of Golgi extract together with tubulin or incorporation of spindle materials rescues Golgi ribbon formation. Therefore, the factors required for postmitotic Golgi ribbon assembly are transferred by the spindle, but the constituents of functional stacks are partitioned independently, suggesting that Golgi inheritance is regulated by two distinct mechanisms. | en |
| dc.identifier.other | 795519922 | en |
| dc.identifier.uri | http://hdl.handle.net/2152.5/732 | en |
| dc.language.iso | en | en |
| dc.subject.mesh | Mitotic Spindle Apparatus | en |
| dc.subject.mesh | Golgi Apparatus | en |
| dc.subject.mesh | Mitosis | en |
| dc.title | The Mitotic Spindle Mediates Inheritance of the Golgi Ribbon Structures | en |
| thesis.date.available | 2012-05-14 | en |
| thesis.degree.discipline | Cell Regulation | en |
| thesis.degree.grantor | Graduate School of Biomedical Sciences | en |
| thesis.degree.level | Ph.D. | en |
| thesis.degree.name | Doctor of Philosophy | en |