Mechanistic analysis of selective inhibition of RNA processing in Escherichia coli
| dc.contributor.advisor | Georgiou, George | en |
| dc.contributor.committeeMember | Iyer, Vishwanath R. | en |
| dc.contributor.committeeMember | Marcotte, Edward M. | en |
| dc.contributor.committeeMember | Meyer, Richard J. | en |
| dc.contributor.committeeMember | Molineux, Ian J. | en |
| dc.creator | Zhou, Li, doctor of microbiology | en |
| dc.date.accessioned | 2013-01-03T21:33:57Z | en |
| dc.date.accessioned | 2017-05-11T22:30:20Z | |
| dc.date.available | 2013-01-03T21:33:57Z | en |
| dc.date.available | 2017-05-11T22:30:20Z | |
| dc.date.issued | 2010-08 | en |
| dc.date.submitted | August 2010 | en |
| dc.date.updated | 2013-01-03T21:34:10Z | en |
| dc.description | text | en |
| dc.description.abstract | In Escherichia coli, the RNA degradosome is a protein complex involved in the general degradation of mRNA and in post-transcriptional gene regulation. The principal components of the degradosome complex are the endoribonuclease RNase E, the phosphorolytic exoribonuclease PNPase, the ATP-dependent RNA helicase RhlB, and the glycolytic enzyme enolase. The RNase E protein is a 1061 amino acid protein which can be divided into three major functional portions: the N-terminal catalytic activity portion; the central membrane anchoring and RNA binding portion; and the C terminal protein-interaction portion which bind to other major degradosome components. RraA and RraB (Regulator of RNase E activity) are protein regulators of RNase E discovered in our lab, which regulate RNase E by binding to the RNase E C-terminal region. The work presented here describes the regulation of rraB gene expression and in vitro studies of degradosome assembly and the effects of RraA/RraB inhibition. rraB is transcribed from its own promoter PrraB. A transposon insertion into glmS encoding glucosamine-6P synthase resulted in a 4 fold increase in the PrraB activity from a PrraB-lacZ fusion the indicating that glmS is serves as a negative regulator of rraB transcription. Consistent with this discovery, real-time RT-PCR revealed that glmS | en |
| dc.description.department | Microbiology | en |
| dc.format.mimetype | application/pdf | en |
| dc.identifier.slug | 2152/ETD-UT-2010-08-1691 | en |
| dc.identifier.uri | http://hdl.handle.net/2152/ETD-UT-2010-08-1691 | en |
| dc.language.iso | eng | en |
| dc.subject | RNA Degradosome | en |
| dc.subject | RNase E | en |
| dc.subject | RraA | en |
| dc.subject | RraB | en |
| dc.title | Mechanistic analysis of selective inhibition of RNA processing in Escherichia coli | en |
| dc.type.genre | thesis | en |