Detecting calcium flux in T cells using a Bayesian model

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dc.contributor.advisorMüller, Peter, 1963 August 9-en
dc.contributor.committeeMemberEhrlich, Laurenen
dc.creatorHu, Zichengen
dc.creator.orcid0000-0002-4168-1725en
dc.date.accessioned2015-11-16T19:58:49Zen
dc.date.accessioned2018-01-22T22:29:10Z
dc.date.available2015-11-16T19:58:49Zen
dc.date.available2018-01-22T22:29:10Z
dc.date.issued2015-08en
dc.date.submittedAugust 2015en
dc.date.updated2015-11-16T19:58:49Zen
dc.descriptiontexten
dc.description.abstractUpon antigen recognition, T cells are activated to carry out its effector functions. A hallmark of T cell activation is the dramatic increase of the intracellular calcium concentration (calcium influx). Indo-1 is a calcium indicator dye widely used to detect T cell activation events in in vitro assays. The use of Indo-1 to detect T cell activation events in live tissues remains a challenge, due to the high noise to signal ratio data generated. Here, we developed a Bayesian probabilistic model to identify T cell activation events from noisy Indo-1 data. The model was able to detect T cell activation events accurately from simulated data, as well as real biological data in which the time of T cell activation events are known. We then used the model to detect OTII T cells that are activated by dendritic cells in thymic medulla in Rip-OVAhi transgenic mouse. We found that dendritic cells contribute 60% of all T cell activations in the mouse model.en
dc.description.departmentStatisticsen
dc.format.mimetypeapplication/pdfen
dc.identifierdoi:10.15781/T2M33Ten
dc.identifier.urihttp://hdl.handle.net/2152/32506en
dc.language.isoenen
dc.subjectBayesianen
dc.subjectMCMCen
dc.subjectT cellsen
dc.subjectTCR signalingen
dc.subjectTwo photon microscopyen
dc.subjectPseudo-prioren
dc.titleDetecting calcium flux in T cells using a Bayesian modelen
dc.typeThesisen

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