BioA and lysA: possible metabolic requirements for pathogenicity of Shigella flexneri

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dc.contributor.advisorPayne, Shelley M.en
dc.contributor.committeeMemberO'Halloran, Terryen
dc.creatorCoughlin, Laura Annen
dc.date.accessioned2010-08-30T20:53:25Zen
dc.date.accessioned2010-08-30T20:53:30Zen
dc.date.accessioned2017-05-11T22:20:09Z
dc.date.available2010-08-30T20:53:25Zen
dc.date.available2010-08-30T20:53:30Zen
dc.date.available2017-05-11T22:20:09Z
dc.date.issued2009-12en
dc.date.submittedDecember 2009en
dc.date.updated2010-08-30T20:53:30Zen
dc.descriptiontexten
dc.description.abstractShigella flexneri is a Gram negative facultative anaerobe that infects millions world-wide each year. The route for infection of a host is through the intestinal and rectal epithelium layers, but it also can survive in the environment. Different genes have been found to be up regulated depending upon its presence in the intracellular or extracellular environment, as shown in previous work in the lab. This thesis seeks to examine the role these upregulated genes, bioA and lysA, play in the intracellular activity of S. flexneri. Knock-out mutations in the bioA and lysA genes were created using P1 transduction. To test the effects of these mutations on S. flexneri, plaque, invasion, and attachment assays were performed. It was found that the bioA mutation resulted in fewer plaques being formed, while the lysA mutation resulted in slower forming and incompletely lysed plaques being formed.en
dc.format.mimetypeapplication/pdfen
dc.identifier.urihttp://hdl.handle.net/2152/ETD-UT-2009-12-393en
dc.language.isoengen
dc.subjectlysAen
dc.subjectShigella flexnerien
dc.subjectbioAen
dc.subjectintracellular virulenceen
dc.titleBioA and lysA: possible metabolic requirements for pathogenicity of Shigella flexnerien
dc.type.genrethesisen

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