Early markers of breast cancer in nipple aspirate fluid

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dc.contributor.advisorLee-Jane W Luen_US
dc.contributor.committeeMemberSuzanne AW Fuquaen_US
dc.contributor.committeeMemberKarl E Andersonen_US
dc.contributor.committeeMemberJonanthan Warden_US
dc.contributor.committeeMemberAnthony M Haagen_US
dc.contributor.committeeMemberAlexander Kuroskyen_US
dc.creatorYafei Huangen_US
dc.date.accessioned2011-12-20T16:05:06Z
dc.date.accessioned2014-02-19T22:05:27Z
dc.date.available2008-06-17en_US
dc.date.available2011-12-20T16:05:06Z
dc.date.available2014-02-19T22:05:27Z
dc.date.created2007-07-27en_US
dc.date.issued2007-07-12en_US
dc.description.abstractNipple aspirate fluid (NAF) refers to the small amount of secretion that is found in breast ducts/lobules of most non-lactating women. This fluid can be collected repeatedly and non-invasively via the nipple using a modified breast pump, and therefore, it is considered to be a potential source for identifying markers of breast cancer. The purpose of this study was to understand factors associated with the ability to secrete fluid and factors associated with the major protein profiles in NAF; and to identify protein profiles of NAF in a group of healthy non-lactating women who were 30-40 years old, not pregnant, not breastfeeding, and not taking contraceptive medications.\r\n\r\nAmong 238 women studied, 66% were secretors of NAF. Using multivariate logistic regression models, higher dietary intake of lactose [Odds Ratio (OR)=2.7; 95% Confidence Interval (CI): 1.5-4.8], earlier menarche (OR=0.8, CI: 0.7-1.0), being parous (OR=2.3, CI: 1.0-5.6), and older at first childbirth (OR=1.5, CI: 1.0-2.1) were found to be independent and positive predictors for being a secretor of NAF. These findings suggest that dietary intake of lactose, a modifiable factor, may be used to change the NAF secretor status of women. \r\n\r\nNAF were analyzed for major proteins. Two major types of protein profiles, type I and type II, were identified. Type I NAF contains proteins found in cystic disease fluid of the breast, whereas type II NAF is enriched in milk-associated proteins. Using multiple logistic regression, type I NAF was predicted independently (P<0.05) by higher body fat mass (Odds Ratio=3.0; CI: 1.5-6.1), more years since last childbirth (OR=2.6; 95% CI: 1.3-5.2) and a higher percentage of calories from saturated fat (OR=4.1; 95% CI: 1.1-14.6). These results suggest that protein profiles of NAF might be influenced by amounts and/or types of dietary and body fat. \r\n\r\nTwo different analytical strategies, 2D gel analysis coupled with MALDI-TOF/TOF, and 1D gel coupled with LC-MS/MS, were used to characterize protein profiles of type I and II NAF. Using these two strategies, a total of 99 proteins were identified: 13 unique to type I NAF, 57 unique to type II NAF, and 29 common to both types. These strategies will be used to characterize proteins in NAF of breast cancer cases. \r\nen_US
dc.format.mediumelectronicen_US
dc.identifier.otheretd-07272007-153218en_US
dc.identifier.urihttp://hdl.handle.net/2152.3/194
dc.language.isoengen_US
dc.rightsCopyright © is held by the author. Presentation of this material on the TDL web site by The University of Texas Medical Branch at Galveston was made possible under a limited license grant from the author who has retained all copyrights in the works.en_US
dc.subjectproteomicsen_US
dc.subjectbreast secretionen_US
dc.subjectbiomarkeren_US
dc.titleEarly markers of breast cancer in nipple aspirate fluiden_US
dc.type.genredissertationen_US
dc.type.materialtexten_US
thesis.degree.departmentPreventative Medicine and Community Healthen_US
thesis.degree.grantorThe University of Texas Medical Branchen_US
thesis.degree.levelDoctoralen_US
thesis.degree.namePhDen_US

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