Transcriptional regulation and chromatin remodeling mechanisms at PHO5

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dc.contributorKladde, Michael
dc.creatorCarvin, Christopher Dumas
dc.date.accessioned2005-08-29T14:35:15Z
dc.date.accessioned2017-04-07T19:49:53Z
dc.date.available2005-08-29T14:35:15Z
dc.date.available2017-04-07T19:49:53Z
dc.date.created2003-05
dc.date.issued2005-08-29
dc.description.abstractRegulation of gene expression is vital for proper growth and prevention of disease states. In eukaryotes this regulation occurs in the context of chromatin which creates an inherent barrier for the binding of trans-acting factors, such as transcription factors and RNA polymerase. This dissertation focuses on the role of transcriptional activators and chromatin remodeling coactivators in the regulation of the repressible acid phosphatase gene PHO5. Our studies show that histone methylation at lysine 4 of histone H3 is required for the full repression of PHO5and GAL1-10. We show that bromodomains, a domain conserved in chromatin remodeling coactivators, may function to stabilize binding. Finally, we present a strategy using DNA methyltransferases as in vivo probes to detect DNA-protein interactions and examine chromatin structure. We extend this strategy to zinc-finger proteins which can be engineered to bind to any desired DNA sequence as a means of targeting methylation with potential use in epigenetic silencing.
dc.identifier.urihttp://hdl.handle.net/1969.1/2193
dc.language.isoen_US
dc.publisherTexas A&M University
dc.subjectchromatin
dc.subjectSet1
dc.subjectbromodomain
dc.subjectPHO5
dc.subjecttargeted DNA methylation
dc.titleTranscriptional regulation and chromatin remodeling mechanisms at PHO5
dc.typeBook
dc.typeThesis

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