The role of reactive oxygen species as mediators of respiratory syncytial virus induced pulmonary inflammation

Simple item page
dc.contributor.advisorRoberto Garofalo M.D.en_US
dc.contributor.committeeMemberXiaodong Chengen_US
dc.contributor.committeeMemberMary Treinen-Moslenen_US
dc.contributor.committeeMemberKent Pinkerton Ph.D.en_US
dc.contributor.committeeMemberIstvan Boldoghen_US
dc.creatorShawn Monique Castroen_US
dc.date.accessioned2011-12-20T16:05:16Z
dc.date.accessioned2014-02-19T22:05:32Z
dc.date.available2010-09-28en_US
dc.date.available2011-12-20T16:05:16Z
dc.date.available2014-02-19T22:05:32Z
dc.date.created2008-08-26en_US
dc.date.issued2008-06-27en_US
dc.description.abstractRespiratory Syncytial Virus (RSV) is the leading cause of epidemic respiratory tract illness in children worldwide. Although the mechanisms of RSV-induced airway disease are unknown, experimental evidence suggests that early local inflammatory processes serve as major initiating events in the pathogenesis of RSV-induced lung disease. RSV induced inflammation is mediated in part by small inflammatory chemokines. We investigated the mechanism of RSV-induced chemokine RANTES gene expression in epithelial cells and identified reactive oxygen species as critical signaling molecules involved in STAT and IRF transcription factor activation and the IKK-epsilon pathway, two activated pathways involved in the regulation of pro-inflammatory gene expression. We showed that RSV induced oxidative stress in vivo and that antioxidant therapy with butylated hydroxyanisole (BHA) attenuated RSV induced oxidative stress, pulmonary inflammation and airway hyper-responsiveness. The one caveat to anti-inflammatory antioxidant therapy was the slight increase in RSV viral load observed following antioxidant administration. To eliminate the undesired outcome of increased viral load, a combination therapy approach was utilized employing BHA and the potent anti-viral IFN-alpha. Combination therapy yielded similar results of diminishing RSV-induced pulmonary inflammation while also decreasing RSV viral load in the lungs. Another key mediator regulated by oxidative stress and involved in inflammation is Poly (ADP)-Ribose Polymerase (PARP). We demonstrated that RSV is a potent inducer of PARP activity and that pharmacological inhibition of PARP with INO-1001 abolished RSV-induced PARP activity. INO-1001 also significantly reduced RSV-induced release of inflammation and lung pathology. Of environmental significance, cigarette smoke is also a potent oxidant mixture and important risk factor for the severity of RSV-induced disease. The mechanism(s) causing a worsening of RSV-induced lung disease by environmental tobacco exposure are unclear. Therefore, we investigated the effect of co-exposure of airway epithelial cells to cigarette smoke condensate (CSC) and RSV on inflammatory chemokine gene expression. We demonstrated that CSC and RSV synergistically increased MCP-1 and IL-8 chemokine expression through NF-kappaB and IRF transcription activation. Overall, the modulation of RSV-induced oxidative processes, either by dampening ROS production through pharmacological intervention or by heightening it by toxicant exposure, identify ROS as major signaling molecules involved in regulating RSV-induced inflammation.en_US
dc.format.mediumelectronicen_US
dc.identifier.otheretd-08262008-113347en_US
dc.identifier.urihttp://hdl.handle.net/2152.3/219
dc.language.isoengen_US
dc.rightsCopyright © is held by the author. Presentation of this material on the TDL web site by The University of Texas Medical Branch at Galveston was made possible under a limited license grant from the author who has retained all copyrights in the works.en_US
dc.subjectRSVen_US
dc.subjectreactive oxygen speciesen_US
dc.subjectPARPen_US
dc.subjectoxidative stressen_US
dc.subjectNF-kappa Ben_US
dc.subjectIRFen_US
dc.subjectinflammationen_US
dc.subjectcigarette smokeen_US
dc.subjectchemokineen_US
dc.subjectantioxidanten_US
dc.titleThe role of reactive oxygen species as mediators of respiratory syncytial virus induced pulmonary inflammationen_US
dc.type.genredissertationen_US
dc.type.materialtexten_US
thesis.degree.departmentPediatricsen_US
thesis.degree.grantorThe University of Texas Medical Branchen_US
thesis.degree.levelDoctoralen_US
thesis.degree.namePhDen_US

Files

Collections

University of Texas Medical Branch