Enzymatic synthesis of CoA derivatives using a new ATP regeneration system

dc.contributor.advisorWillets, Katherine A.en
dc.contributor.advisorKeatinge-Clay, Adrian Tristanen
dc.creatorWilliams, Diana, 1966-en
dc.date.accessioned2011-01-06T20:42:56Zen
dc.date.accessioned2011-01-06T20:43:02Zen
dc.date.accessioned2017-05-11T22:21:03Z
dc.date.available2011-01-06T20:42:56Zen
dc.date.available2011-01-06T20:43:02Zen
dc.date.available2017-05-11T22:21:03Z
dc.date.issued2010-08en
dc.date.submittedAugust 2010en
dc.date.updated2011-01-06T20:43:02Zen
dc.descriptiontexten
dc.description.abstractThe research project, The Enzymatic Synthesis of CoA Derivatives Using a New ATP Regeneration System, describes the multiple lab trials conducted to develop an ATP regeneration system using various concentrations of specific substrates and the two enzymes MatB and PrpE. Reactions were combined using different concentrations of ADP, the addition or removal of ADK (adenylate kinase), and the substitution of either MatB or PrpE. Further reactions were combined using trans-crotonyl, trifluoroacetic acid, butyric acid, acetic acid, and creatine phosphokinase. This report also includes the methods used and analysis of the different chromatographs of each sample tested.en
dc.description.departmentScience and Mathematics Educationen
dc.format.mimetypeapplication/pdfen
dc.identifier.urihttp://hdl.handle.net/2152/ETD-UT-2010-08-1721en
dc.language.isoengen
dc.subjectEnzymatic synthesisen
dc.subjectCoA derivativesen
dc.subjectATP regeneration systemen
dc.titleEnzymatic synthesis of CoA derivatives using a new ATP regeneration systemen
dc.type.genrethesisen

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