Identification of regulators of heat shock-inducible gene in Arabidopsis

Simple item page
dc.contributor.committeeChairShi, Huazhong
dc.contributor.committeeMemberXie, Zhixin
dc.contributor.committeeMemberPaul, Pare
dc.creatorRuan, Qing Xia
dc.date.accessioned2016-11-14T23:36:45Z
dc.date.available2013-01-24T20:17:03Z
dc.date.available2016-11-14T23:36:45Z
dc.date.issued2012-12
dc.description.abstractBy using forward genetic screening, we identified an Arabidopsis mutant LL729 showing reduced expression of the luciferase reporter gene driven by the heat stress inducible promoter of AtHsp18.2 gene. Extensive phenotyping of the LL729 mutant revealed that LL729 is resistant to the toxic non-selected herbicide methyl viologen. Through positional cloning, we found that the mutation responsible for the LL729’s luciferase imaging and methyl viologen resistant phenotypes is localized in At5g05630 encoding a polyamine uptake transporter named PUT3. We deduce that the mutation resulting in a change of ser252 to asn252 in the PUT3 reduced the transport of methyl viologen as well as polyamines into Arabidopsis.
dc.format.mimetypeapplication/pdf
dc.identifier.urihttp://hdl.handle.net/2346/47480
dc.language.isoeng
dc.rights.availabilityUnrestricted.
dc.subjectHsp18.2
dc.subjectPolyamine uptake transporter 3
dc.subjectMethyl viologen
dc.subjectLuciferase
dc.titleIdentification of regulators of heat shock-inducible gene in Arabidopsis
dc.typeThesis

Files

Collections

Texas Tech University