Characterization of VIBRIO Parahaemolyticus-induced Intestinal inflammation in the mouse

Simple item page
dc.contributor.advisorOrth, Kimen
dc.creatorLee, Oliviaen
dc.date.accessioned2010-07-12T18:49:37Zen
dc.date.accessioned2014-02-19T22:00:22Z
dc.date.available2010-07-12T18:49:37Zen
dc.date.available2014-02-19T22:00:22Z
dc.date.issued2009-09-04en
dc.description.abstractThe Gram-negative marine bacterium Vibrio parahaemolyticus is a leading cause of gastroenteritis from the consumption of contaminated seafood. Some recent outbreaks are attributed to a rise in ocean temperatures, a trend that is likely to continue as a result of global warming and emphasizes the need to study the virulence mechanisms of the pathogen. V. parahaemolyticus utilizes a type III secretion system to inject effectors that disrupt signal transduction in eukaryotic hosts during infection. Herein, we describe an in vivo model of infection by V. parahaemolyticus using germfree mice. V. parahaemolyticus is recovered from the cecum and colon of mice infected by oral gavage. Mice infected with a pathogenic, wild type strain of V. parahaemolyticus exhibit intestinal inflammation characterized by epithelial damage, submucosal edema, crypt abscess and hyperplasia, and infiltration of the lamina propria by neutrophils. Using mutant strains of V. parahaemolyticus, we have determined that thermostable direct hemolysin, the most well characterized virulence factor produced by V. parahaemolyticus, and T3SS2, one of the two type III secretion systems present in this bacterium, both contribute to enteropathogenesis. Analysis of the expression of inflammatory cytokines IL-1? and IFN-gamma and chemokines KC, MIP-2 alpha, and CXCL-9 reveals that the duration of the inflammatory response is extended in the presence of TDH. In addition to delineating the roles of the virulence factors TDH, type III secretion system 1, and type III secretion system 2, in the infection process, we have developed an experimental system that will enable further characterization of type III effectors of V. parahaemolyticus.en
dc.format.digitalOriginborn digitalen
dc.format.mediumElectronicen
dc.format.mimetypeapplication/pdfen
dc.identifier.other760314871en
dc.identifier.urihttp://hdl.handle.net/2152.5/681en
dc.language.isoenen
dc.subjectVibrio parahaemolyticusen
dc.subjectVibrio Infectionsen
dc.subjectIntestinal Diseasesen
dc.titleCharacterization of VIBRIO Parahaemolyticus-induced Intestinal inflammation in the mouseen
dc.type.genredissertationen
dc.type.materialTexten
thesis.date.available2011-09-04en
thesis.degree.departmenten
thesis.degree.disciplineBiological Chemistryen
thesis.degree.grantorGraduate School of Biomedical Sciencesen
thesis.degree.levelPh.D.en
thesis.degree.nameDoctor of Philosophyen

Files

Collections

University of Texas Southwestern Medical Center