Enzymatic inhibition-based biosensing on nitrogen-doped carbon nanotube electrodes

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dc.contributor.advisorStevenson, Keith J.en
dc.contributor.committeeMemberWebb, Lauren Jen
dc.creatorRust, Ian Matthiasen
dc.date.accessioned2015-10-16T18:19:02Zen
dc.date.accessioned2018-01-22T22:28:29Z
dc.date.available2015-10-16T18:19:02Zen
dc.date.available2018-01-22T22:28:29Z
dc.date.issued2015-05en
dc.date.submittedMay 2015en
dc.date.updated2015-10-16T18:19:02Zen
dc.descriptiontexten
dc.description.abstractWhile previous work has demonstrated the effectiveness of nitrogen-doped carbon nanotubes (N-CNTs) as biogenic electrode materials in first- and second-generation biosensors, this thesis primarily explores enzymatic inhibition-based biosensing schemes on N-CNT electrodes. This type of scheme enables the detection of enzymatic inhibitors, as opposed to enzymatic substrates, making these inhibition-based biosensors much more suitable for the monitoring of environmental pollutants. Presented in this thesis is a biosensor which couples N-CNTs with glucose oxidase (GOx) through spontaneous physical adsorption for the highly sensitive detection of aqueous silver ions. Included is a thorough discussion of the parameters that affect response time as well the biosensor’s aptitude for repeated use. A later chapter presents initial work towards the inhibition-based detection of sucralose, a relatively new environmental pollutant. A bi-enzymatic approach is explored, in which both GOx and invertase are immobilized on an N-CNT modified electrode. Finally, shifting focus from inhibition-schemes, the last remaining chapter investigates the coupling of CNTs and N-CNTs with methylene green (MG), a redox mediator used in second-generation biosensors based on NADH oxidation. Common coupling techniques are examined for their effectiveness in decreasing the overpotential required for NADH oxidation.en
dc.description.departmentChemistryen
dc.format.mimetypeapplication/pdfen
dc.identifierdoi:10.15781/T2CC9Pen
dc.identifier.urihttp://hdl.handle.net/2152/31752en
dc.subjectCarbon nanotubesen
dc.subjectNitrogen-dopeden
dc.subjectCNTen
dc.subjectN-CNTen
dc.subjectGlucose oxidaseen
dc.subjectGOxen
dc.subjectMethylene greenen
dc.subjectAdsorptionen
dc.subjectEnzyme inhibitionen
dc.subjectSensoren
dc.subjectBiosensoren
dc.subjectInhibition-based biosensoren
dc.subjectSilveren
dc.subjectSucraloseen
dc.subjectInvertaseen
dc.subjectEnvironmental chemistryen
dc.subjectEnvironmenten
dc.subjectAnalyticalen
dc.subjectAnalytical chemistryen
dc.titleEnzymatic inhibition-based biosensing on nitrogen-doped carbon nanotube electrodesen
dc.typeThesisen

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