Development of cotton callus initiation medium and in vitro screening for osmotic stress tolerance
| dc.creator | Zimmerman, Thomas W | |
| dc.date.accessioned | 2016-11-14T23:21:25Z | |
| dc.date.available | 2011-02-18T21:23:03Z | |
| dc.date.available | 2016-11-14T23:21:25Z | |
| dc.date.issued | 1987-05 | |
| dc.description.abstract | Asceptic excised seed hypocotyls of the Gossypium hirsutum L. varieties Acala 1517-75, Coker 500, Dunn 120, Paymaster 303, and TMl, and the exotic cotton lines, Tl, T25, and T133, were cultured for 24 days on six media and two gelling agents, Difco bacto agar and Kelco Gelrite. Significantly more callus was initiated on media solidified with Gelrite than with agar. The Linsmaier and Skoog medium (21) supplimented with 30 g/1 glucose, 0.1 mg/1 BA, and 0.1 mg/1 2,4-D supported significantly more callus production than the other five media. Exotic cottons, representing the seven races, were subjected to polyethylene glycol (PEG) induced osmotic stress in vitro in modified Linsmaier and Skoog (21) medium. The PEG levels were 0, 10, 20, and 30% w/v representing osmotic stresses of -5, -10, -16, and -24 bars respectively- The rate of dry matter accumulation, measured as dry cell weight (DCW), varied among cotton lines at each PEG level. The differences in density, DCW divided by packed cell volume, among the cotton cell lines at the stress levels indicate osmotic adjustment by the cells. | |
| dc.format.mimetype | application/pdf | |
| dc.identifier.uri | http://hdl.handle.net/2346/16073 | en_US |
| dc.language.iso | eng | |
| dc.publisher | Texas Tech University | en_US |
| dc.rights.availability | Unrestricted. | |
| dc.subject | Callus (Botany) | en_US |
| dc.subject | Cottonseed -- Testing | en_US |
| dc.subject | Plants -- Effect of stress on | en_US |
| dc.title | Development of cotton callus initiation medium and in vitro screening for osmotic stress tolerance | |
| dc.type | Thesis |