Post-Transcriptional Regulation by microRNAS in Pregnancy and Parturition
| dc.contributor.advisor | Mendelson, Carole R. | en |
| dc.creator | Renthal, Nora Edwards | en |
| dc.date.accessioned | 2012-08-15T14:29:13Z | en |
| dc.date.accessioned | 2014-02-19T22:03:32Z | |
| dc.date.available | 2012-08-15T14:29:13Z | en |
| dc.date.available | 2014-02-19T22:03:32Z | |
| dc.date.issued | 2012-08-15 | en |
| dc.description.abstract | Throughout most of pregnancy, uterine quiescence is maintained by increased progesterone receptor (PR) transcriptional activity, while spontaneous labor is initiated/facilitated by a concerted series of biochemical events that activate inflammatory pathways and negatively impact PR function. In this study, we uncovered a new regulatory pathway whereby miRNAs serve as hormonally-modulated and conserved mediators of contractile gene regulation in the pregnant uterus from mouse to human. Using miRNA and gene expression microarray analyses of uterine tissues, we identified a conserved family of miRNAs, the miR-200 family, that is highly induced at term in both mice and humans, as well as two coordinately downregulated targets, zinc finger E-box binding homeobox proteins, ZEB1 and ZEB2, which act as transcriptional repressors. We also observed upregulation of the miR-200 family and downregulation of ZEB1 and ZEB2 in two different mouse models of preterm labor. We further demonstrated that ZEB1 is directly upregulated by the action of P4/PR at the ZEB1 promoter. Excitingly, we observed that ZEB1 and ZEB2 inhibited expression of the contraction-associated genes, oxytocin receptor and connexin-43 and blocked oxytocin-induced contractility in human myometrial cells. Together, these findings implicate the miR-200 family and their targets ZEB1 and ZEB2 as novel P4/PR-mediated regulators of uterine quiescence and contractility during pregnancy and labor, and shed new light on the molecular mechanisms involved in preterm birth. [Keywords: microRNA; pregnancy; parturition; uterus; myometrium; progesterone; hormonal regulation; ZEB1; ZEB2; miR-200] | en |
| dc.identifier.other | 812607576 | en |
| dc.identifier.uri | http://hdl.handle.net/2152.5/1113 | en |
| dc.language.iso | en | en |
| dc.subject | Labor, Obstetric | en |
| dc.subject | MicroRNAs | en |
| dc.subject | Transcription Factors | en |
| dc.title | Post-Transcriptional Regulation by microRNAS in Pregnancy and Parturition | en |
| dc.type | Thesis | en |
| thesis.date.available | 2012-07-10 | en |
| thesis.degree.discipline | Genetics and Development | en |
| thesis.degree.grantor | Graduate School of Biomedical Sciences | en |
| thesis.degree.level | Ph.D. | en |
| thesis.degree.name | Doctor of Philosophy | en |