The effects of a trichloromelamine wash on Escherichia coli O157:H7 and Salmonella spp. prevalence on beef hides
dc.creator | Sultemeier, Crystal Michele | |
dc.date.accessioned | 2016-11-14T23:16:50Z | |
dc.date.available | 2011-02-18T20:45:50Z | |
dc.date.available | 2016-11-14T23:16:50Z | |
dc.date.issued | 2003-12 | |
dc.degree.department | Animal Science | en_US |
dc.description.abstract | In a multi-stage experiment, the efficacy of Trichloromelamine (TCM), a chlorine-containing disinfectant compound, on the reduction of Escherichia coli 0157:H7 and Salmonella spp. on beef hides at an abattoir was determined. Twenty head of cattle were sampled on each of two consecutive days of each week during a six-week period. A preliminary swab was taken from each head immediately following exsanguinations. Cattle were then sprayed with TCM and a second swab was taken immediately prior to hide removal. Swabs were then analyzed for prevalence of E. coli 0157:H7 and Salmonella spp.. In a second trial, swabs were taken both prior to and following treatment. Treatment in this trial included TCM and TCM plus a thickening agent (TCM + T), with ten head receiving each treatment. In a third experiment, individual hide samples were spread with fecal slurries which were inoculated with K coli 0157:H7 and Salmonella spp.. Hide samples were sprayed with TCM or TCM + T and allowed a dwell time of 1 to 10 minutes. E. coli and Salmonella spp. were then enumerated for each hide sample. In the first trial, significant decreases were seen for E. coli 0157JI7 (P < 0.0002) and Salmonella spp. (P < 0.0001). No significant decreases were observed for E. coli 0157:H7 in the second trial due to a small number of preliminary positives. Significant decreases in Salmonella spp. were seen from TCM and TCM + T treatments (P < 0.0001). A significant difference was also observed between TCM and TCM + T treatments with TCM + T having a greater reduction (P < 0.05). In the enumeration study, significant log reduction was not seen for E coli 0157:H7 for either treatment. Both treatments exhibited a significant log reduction (P < 0.0001). Results of this study indicate TCM is an effective in-slaughter means of microbial intervention. | |
dc.format.mimetype | application/pdf | |
dc.identifier.uri | http://hdl.handle.net/2346/14669 | en_US |
dc.language.iso | eng | |
dc.publisher | Texas Tech University | en_US |
dc.rights.availability | Unrestricted. | |
dc.subject | Escherichia coli O157:H7 | en_US |
dc.subject | Beef industry -- Health aspects -- Sanitation | en_US |
dc.subject | Cattle trade | en_US |
dc.subject | Salmonella | en_US |
dc.title | The effects of a trichloromelamine wash on Escherichia coli O157:H7 and Salmonella spp. prevalence on beef hides | |
dc.type | Thesis |