Blood and water; the archaeological excavation and historical analysis of the Wreck of the Industry, a North-American transport sloop chartered by the British army at the end of the Seven Years' War: British colonial navigation and trade to supply Spanish Florida in the eighteenth century
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In the 10-mer RNA duplex model system a 4-isocyano TEMPO spin-label is individually attached to one strand and two strands are annealed to measure distances. This methodology is limited to systems in which two oligonucleotides are annealed together. To circumvent this limitation and also to explore single-strand dynamics a new methodology was implemented, double spin-labeling. Double spin-labeled single-stranded RNA was investigated as a single-strand and within a duplex via MALDI-TOF-MS, EPR spectroscopy and RP-HPLC. A double spin-labeling strategy in this work will be applicable to large complex RNAs like Group I intron of Tetrahymena thermophilia. Captain Daniel Lawrence, was one of four sloops detailed to serve as a transport to supply the British Florida garrisons. The Industry ran aground on the bar outside of St. Augustine's harbour on May 6, 1764. The transport was carrying six-pound cannons, ammunition and artificer's tools. Further investigation of documents describing eighteenth-century trade and shipping to St. Augustine led to the discovery that the Lawrence family of sea captains provided a vital link between British New York and Spanish St. Augustine. An examination of the materials recovered from Site 8SJ3478 sheds light on exactly what a particular vessel carried during a period of transition in Florida's history.