Browsing by Subject "wastewater"
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Item 16S rRNA-Based Tag Pyrosequencing of Complex Food and Wastewater Environments: Microbial Diversity and Dynamics(2012-02-14) McElhany, KatherineEnvironmental microbiology has traditionally been performed using culture-based methods. However, in the last few decades, the emergence of molecular methods has changed the field considerably. The latest development in this area has been the introduction of next-generation sequencing, including pyrosequencing. These technologies allow the massively parallel sequencing of millions of DNA strands and represent a major development in sequencing technologies. The purpose of this study was to use both pyrosequencing and traditional culture-based techniques to investigate the diversity and dynamics of bacterial populations within milk and untreated sewage sludge samples. Pasteurized and raw milk samples were collected from grocery stores and dairies within Texas. Milk samples were analyzed by plating, pyrosequencing, and an assay for the presence of cell-cell signaling molecules. Samples were processed, stored, and then evaluated again for spoilage microflora. The results of this study showed that raw milk had a considerably higher bacterial load, more diversity between samples, and a significantly higher concentration of pathogens than pasteurized milk. Additionally, this study provided evidence for varying spoilage microflora between raw and pasteurized milk, as well as evidence for the production of cell-cell signaling molecules by bacterial organisms involved in milk spoilage. Four samplings of untreated sewage sludge were collected from wastewater treatment plants in seven different municipalities across the United States. Samples were subjected to quantification of selected bacterial organisms by culture and a pyrosequencing analysis was performed on extracted community DNA. The results of this study showed that untreated sewage sludge is inhabited by a huge diversity of microorganisms and that certain municipalities may have distinct bacterial populations that are conserved over time. Additionally, this study provided some evidence for seasonal differences in several of the major bacterial phyla. Lastly, this study emphasized the challenges of comparing results obtained by culture and pyrosequencing. In conclusion, this study showed that both milk and sewage are highly diverse, dynamic environments that can contain organisms of public health concern. The use of both culture-based methods and pyrosequencing in this study proved a complementary approach, providing a more comprehensive picture of both microbial environments.Item Characterization of wastewater subsurface drip emitters and design approaches concerning system application uniformity(2009-06-02) Duan, XiaojingSubsurface drip distribution is an important on-site wastewater treatment technique which is widely used with various soil types and restricted site conditions. It can distribute pretreated wastewater uniformly into soil. Some recent field applications showed low application uniformities, which was reflected in overloading of the field near the supply manifold while low emitter discharge rates occurred at the end of lateral. Designers are seeking appropriate operation pressures and drip zone configurations to improve system application uniformity. This research was conducted to test some popular wastewater drip products in both lab and field-scale experiments. The first goal of this study was to evaluate the performance of five subsurface drip products under eight operational pressures ranging from 0 to 310 kPa (45 psi). After evaluation of each group of 60 emitters, results showed that Netafim Bioline pressure compensating (PC) emitters exhibited a uniformity coefficient (UC) of 95% with a coefficient of variance (Cv) of 4.9%. The average UC of Geoflow Wasteflow products is 94.4% and Cv value is 6.8%. Flow rate and pressure relationships (Q-H curves) were developed for each drip emitter tested. By analyzing low and normal operational pressure ranges, Q-H curves were fitted to the data and resulted in R2 values ranging from 1.000 to 0.414. Geoflow pressure compensating products possess the features of non-pressure compensating emitters under low pressure head. Netafim PC products are characterized as pressure compensating over the full range of operational pressures and emit water with nominal uniformity during low pressure range. To evaluate drip zone configurations with respect to distribution uniformity, a field-scale experiment was set up and three drip tubing products were tested in different dosing and operation schemes. Three factors of wastewater drip system design were tested. System operation pressure (138 kPa/20 psi and 276 kPa/40 psi); different pressure control components (pressure regulator/recirculation valve) and schemes (continuous flushing/intermittent flushing); and supply line length (7.6 m/25 ft, 15.2 m/50 ft, and 30.4 m/100 ft) were evaluated to compare their influence on water application uniformity. It was concluded that, for Geoflow PC and NPC products, among all three factors, system operational pressure has the greatest effect on drip system application uniformity; supply line length has the least influence. For Netafim PC tubing, pressure control scheme has the greatest effect on drip system application uniformity; supply line length has the least influence. The optimal combination of the three factors could save more than 10 minutes of dosing time to meet the required dosing application uniformity. An engineering computation example on system fill time was presented and compared to experimental results to demonstrate the possible gap between typical design processes and real field application.Item Comparison of the Prevalence and Genotypic Characteristics of Clostridium difficile in a Closed and Integrated Human and Swine Population in Texas(2011-10-21) Norman, Keri NoelleClostridium difficile has been recognized as one of the leading causes of nosocomial diarrhea and pseudomembranous colitis in human hospitals and nursing homes since the 1970s; however, recent occurrences of community-acquired cases have led researchers to search for additional sources of these infections. Some of the possible sources being investigated include food animals and retail meat. The objective of this study was to compare the prevalence and genotypic characteristics of C. difficile isolated from a closed population in Texas consisting of both humans and swine. Implicit in this objective, we seek to investigate the possible food safety and occupational risks associated with swine and C. difficile. Isolation of C. difficile was performed utilizing an enrichment technique and restrictive media. Polymerase chain reaction (PCR) was used to test for the presence of the toxin A and B genes, the tcdC gene deletion, and the binary toxin gene. Genotypic characteristics were compared using PCR toxinotyping and pulsed-field gel electrophoresis (PFGE). Antimicrobial susceptibility was tested using commercially available tests (ETest?) for 11 different antibiotics. Statistical comparisons (both parametric and non-parametric, and appropriate to the data) were performed both between and among host species. We tested 2,292 aggregated human wastewater samples and 2,936 swine fecal samples from 2004 to 2006 and found 271 (11.8 percent) and 252 (8.6 percent) to be positive for C. difficile, respectively. The prevalence of C. difficile among swine production groups differed significantly (p<0.05); however, prevalence in the human occupational group cohorts (swine workers and non-workers) did not differ (p=0.81). The majority of the human and swine isolates were a PFGE NAP7 (a variant pattern with 90.5 percent similarity) toxinotype V strain. Antimicrobial resistance levels and multi-resistance patterns were generally similar between host species; however, there was decreased susceptibility (p<0.05) to ampicillin, clindamycin, and imipenem observed in swine isolates, whereas there was decreased susceptibility (p<0.05) to ciprofloxacin in the human isolates. The similarity in C. difficile prevalence between swine workers and non-workers suggests a low occupational hazard of working with swine as it relates to C. difficile source. We also found that there is a decreased prevalence of C. difficile in late production groups in swine suggesting a lowered risk of food-borne exposure. However, the majority of the isolates derived from the human wastewater and swine appeared to be of very similar strain types, suggesting that a common environmental point source predominates for both hosts.Item Effects of Anthropogenic Nutrient Enrichment on Exotic and Restored Native Aquatic Vegetation(2012-07-16) Parnell, AllisonUnderstanding how nutrient input into coastal wetlands influences aquatic vegetation and the fate of anthropogenic nutrient inputs can help improve water quality management plans. The goals of this study were to (1) compare nutrient concentrations in various storage compartments downstream from two point nutrient sources (a sewage treatment plant outfall and a residential detention basin) in Armand Bayou, a coastal brackish wetland in the Galveston Bay (Texas, USA) watershed, and (2) determine if nutrient storage in those compartments helped improve water quality downstream. Water column nutrients can be assimilated by aquatic vegetation, adsorbed to sediment, or diluted within the system as distance from source input increases. To determine the fate of nutrients to Armand Bayou, I measured nutrient concentrations in the sediment, water column, pore water, and tissue of exotic and restored native plants downstream from a sewage treatment plant and a residential detention basin. To assess nutrient removal potential of a common exotic species, I determined relative growth and nutrient uptake rates of Eichhornia crassipes. Water column total nitrogen, NH?? and NO?? concentrations decreased by 95, 96 and 99 percent downstream from the sewage outfall (~2200 m distance). Water column NH?? and NO?? concentrations decreased by 93 and 75 percent downstream from the detention basin (~2500 m distance). Exotic species Alternanthera philoxeroides, Pistia stratiotes and E. crassipes showed higher aboveground/emergent tissue nutrient content than restored, native Schoenoplectus californicus for both tributaries. Schoenoplectus californicus had the largest biomass although appeared to be limited in its ability to remove nitrogen from the water column. Nutrient uptake rates by E. crassipes were low and did not change with increasing distance from nutrient source, but high relative growth rates in both tributaries suggest the nuisance potential of this exotic species. Low sediment and pore water nutrient concentrations for both tributaries suggest these compartments are not sinks. All plant species did not respond to changes in water column nutrient concentrations with increasing distance from source input suggesting dilution to be the main factor in water column nutrient decline for both tributaries. This study will provide water quality resource managers guidance on the development of total maximum daily loads (TMDLs) for water bodies impaired by high nutrient loading and the implementation of wetland plants efficient in nutrient removal for water quality improvement.Item Effects of UV Light Disinfection on Tetracycline Resistant Bacteria in Wastewater Effluents(2011-10-21) Childress, HannahThe ubiquitous use of antibiotics has led to an increasing number of antibioticresistant bacterial strains, including strains that are multidrug resistant, pathogenic, or both. Numerous studies have been conducted showing the presence of antibioticresistance genes (ARGs) and antibiotic-resistant and multidrug resistant bacteria in wastewater and drinking water treatment plants. There is also evidence to suggest that ARGs spread to the environment, and to humans and animals, through wastewater effluents. The overall objective of this study was to investigate the effect of UV light disinfection on ARGs and antibiotic resistant bacteria. Wastewater effluent samples from a wastewater treatment plant (WWTP) in Texas were evaluated for differences in abundance and diversity of tetracycline resistant bacteria before and after UV treatment. The effects of photoreactivation or dark repair on the reactivation of bacteria present in WWTP effluent after UV disinfection were also examined. Culture based methods were used to characterize viable heterotrophic, tetracycline resistant heterotrophic, E. coli, and tetracycline resistant E. coli bacteria present before and after UV treatment. Molecular methods were used to characterize the diversity of organisms present and to test for the presence of tet(Q), a tetracycline resistance gene associated with human origins. UV disinfection was found to be as effective at reducing concentrations of resistant heterotrophs and E. coli as it was at reducing total bacterial concentrations. The lowest survival ratio following UV disinfection was observed in tetracycline-resistant E. coli, showing it to be particularly susceptible to UV treatment. Photoreactivation and dark repair rates were found to be comparable to each other for all bacterial populations. UV disinfection was found to significantly alter the community composition of tetracycline-resistant bacteria, though it did not have the same effect on the total bacterial community.Item Escherichia coli Regrowth and Macroinvertebrate Health in Urban and Rural Streams(2012-07-16) McCrary, Kathryn JordanOver the last few decades, increased urbanization has led to a new recognition in stream health ? the urban stream or the urban stream syndrome. Understanding urban water quality is important for identifying those factors or sources that contribute to impairment. Many streams are listed as impaired because of the increased concentrations of pathogens. While wastewater treatment plants (WWTPs) discharge effluent that has been disinfected, often downstream from WWTPs point sources are high numbers of indicator bacteria, Escherichia coli. This study collected data on the recovery and regrowth of E. coli by collecting ultraviolet light treated effluent from the Carters Creek WWTP and spiked it with three different concentrations of DOC derived from a leaf and grass extract. Escherichia coli were enumerated at 6, 12, 18, 24, 36, 48, and 72 hours. After 6 h growth for each of the grass treatments, except for the control and high grass treatment exceeded the primary contact recreation standard for surface water quality. At 18h the low and high leaf treatments exceeded the primary contact recreation standard for surface water quality. The chemistry of each flask was analyzed for DOC, total N, NO3-N, NH4-N, Na , K , Mg 2, Ca 2, F-, Cl-, SO4-2 and PO4-3 at t=0 and t=72 h. CNP values for both leaf and grass treatments ranged from 2.22 - 36.5. Regrowth was not observed in those treatments with a CNP value below 5. Biodegradability of the treatments was examined to identify the limiting nutrient. By focusing on reducing the CNP value below 5 of the receiving water, recovery and regrowth of E. coli downstream from WWTPs can be reduced. The biodegradability test suggested that in the presence of excess DOC, N is the limiting nutrient. Certain macroinvertebrate species, Ephermeroptera, Trichoptera, and Plecoptera (EPT), are indicators of good stream health. Macroinvertebrates were collected at nine watersheds within the Bryan/College Station area, a rapidly urbanizing community, upstream and downstream from WWTPs and analyzed for relative abundance of pollution intolerant (percent EPT) and pollution tolerant species. All sites downstream from a WWTP had percent EPT present in the collection.Item Evaluation of the application uniformity of subsurface drip distribution systems(Texas A&M University, 2004-09-30) Weynand, Vance LeoThe goal of this research was to evaluate the application uniformity of subsurface drip distribution systems and the recovery of emitter flow rates. Emission volume in the field, and laboratory measured flow rates were determined for emitters from three locations. Additionally, the effects of lateral orientation with respect to slope on emitter plugging was evaluated. Two different emitters were tested to evaluate slope effects on emitter plugging (type Y and Z). The emitters were alternately spliced together and installed in an up and down orientation on slopes of 0, 1, 2 and 4% and along the contour on slopes of 1 and 2%. The emitters were covered with soil and underwent a simulated year of dosing cycles, and then flushed with a flushing velocity of 0.6 m/s. Initial flow rates for the two emitter types were 2.38 L/hr with a C.V. of 0.07. There was no significant difference in flow rates among slopes for type Y emitters, but there was a significant difference between the 1% and 2 % contour slopes for type Z emitters. Application uniformity of three different laterals at each site was evaluated. Sections of the lateral from the beginning, middle and end were excavated and emission volumes were recorded for each emitter. Application uniformity of laterals ranged from 48.69 to 9.49%, 83.55 to 72.60%, and 44.41 to 0% for sites A, B, and C, respectively. Mean emitter flow rate was 2.21, 2.24, and 2.56 L/hr for sites A, B, and C, respectively under laboratory conditions. Application uniformity under laboratory conditions ranged from 70.97 to 14.91%, 86.67 to 79.99%, and 85.04 to 0.00% for sites A, B, and C, respectively. A flushing velocity of 0.15 m/s with no chlorination, shock chlorination of 3400 mg/L and flushing velocity of 0.15 m/s, and shock chlorination of 3400 mg/L and flushing velocity of 0.6 m/s treatment regiments were applied to all laterals collected to assess emitter flow rate recovery to the nominal flow rate published by the manufacturer. All laterals showed an increase in the number of emitters within 10% of the published nominal flow rate.Item Reactive Transport Modeling of Natural Attenuation in Stormwater Bioretention Cells and Under Land Application of Wastewater(2014-04-29) Zhang, JingqiuNatural attenuation is a cost effective method to treat wastewater applied into soil. The natural attenuation process includes diffusion, dispersion, microbial activity, oxidation, mineral precipitation, sorption, and ion exchange to mitigate hydrocarbon, nutrient, metals, and solids. Vegetation also plays an important role in reducing water volume, and removing nutrients and solutes from the contaminated soil. We used a reactive transport model MIN3P-THM to simulate the natural attenuation on stormwater runoff, and oil and gas produced wastewater. In bioretention systems, the model results indicated that the bioretention systems were able to remove most of heavy metals, nitrate, and organic carbon through natural attenuation in the soil. Due to macropores and fast flow paths created by roots in vegetated cells, the water can carry ions flowing out of the system very quickly leading to a higher outflow rate and less removal efficiency than non-vegetated cells. The model also tested a range of possible design configurations to determine the optimal saturated zone thickness and outlet location for nitrate removal. In addition, different rainfall levels did influence the natural attenuation performance of bioretention cells under long time application. Due to less water and chemical input, climate patterns may lead to better removal of heavy metals. For land application of Oil and Gas Exploration and Production wastewater, five scenarios were developed to study the impact of chloride, salts, and organic matters on natural attenuation. Water and salinity stress were considered in the model to deal with high salinity wastewater in the root zone. For High-FDS and High-Cl treatments, long time application of high salinity wastewater did accumulate salts in the root zone and affect groundwater quality. Under the High-TOC treatment, TOC begins to build up in the root zone in concentrations up to 750 mg/L. More attention should be paid on long-term land application of high salinity wastewater; the application process may pollute groundwater and nearby rivers causing human health hazard. Plants would reduce water uptake to survive themselves when water and solute stress occurred under high salinity conditions.