Browsing by Subject "seasonality"
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Item Changes in Gene Expression of Goat Developing Testes and Sperm During Breeding and Non-breeding Season(2012-07-16) Faucette, AzureTesticular function is fundamental to male fertility, since testicular cells act in collaboration with each other to signal sex differentiation, the initiation of puberty and spermatogenesis. Complications that can be influenced by many factors will affect sperm number, morphology, motility, chromatin quality and acrosomal integrity. The purpose of these studies was to analyze the changes in gene expression in the developing testes and analyze the seasonal changes in gene products in sperm of mature bucks. In the first experiment, testes were harvested from five Alpine bucks at 0, 2, 4, 6, and 8 months of age. Northern and in situ hybridization indicated that the largest change in gene expression occurred during the first 4 months of goat testes development. Sex determining region Y-box 9 (SOX9) and Heat Shock protein A8 (HSPA8) peaked at 2 months of age, and were expressed in Sertoli cells and spermatogonium, respectively. At 4 months, expression of Stimulated by Retinoic Acid gene 8 (STRA8), Protamine1 (PRM1) and Outer Dense Fiber protein 2 (ODF2) was strongly up-regulated in early and maturing germ cells, respectively. In the second experiment, RNA from ejaculated spermatozoa collected from mature Alpine bucks in peak (October) and non-peak (April) breeding season were analyzed on a 4 x 44K Agilent bovine microarray. One thousand three hundred and eighteen gene products were differentially expressed 2-fold or more (p ? 0.05 ) was expressed in mature goat sperm collected October and April. To eliminate the likelihood of false positives, the cut off was set to fold change of 3 or more at p ? 0.01 which narrowed the list of genes to 50 transcripts. Real time PCR results confirmed the expression of Sperm Adhesion Molecule 1 (SPAM1) in April, and the expression of Glycerol kinase 2(GK2) and Myc Binding Protein 2 (MYCBP2) in October. Based on the results from both experiments, it can be concluded that: SOX9 and HSPA8 expression play an important role in tubular formation and germ cell maintenance; two months after SOX9 and HSPA8 expression, genes that are associated with spermatogenesis initiation and completion are upregulated; and validation of the seasonal changes in sperm mRNA levels may provide additional insight to testicular events as they relate to breeding and non-breeding season.Item Interactive Roles of Gonadotropin-Releasing Hormone and RF-Amide Related Peptide 3 in Adenohypophyseal Physiology and Reporduction in the Mare(2013-01-28) Thorson, Jennifer FrancesThe seasonal termination of ovarian cycles in mares initiated near the time of the autumnal equinox is a significant managerial issue for horse breeders world-wide. Studies presented herein had two over-arching aims. In Aim I, objectives were to develop the principals needed to apply gonadotropin-releasing hormone (GnRH) therapeutics for routinely establishing pregnancies in the winter anovulatory mare. We first tested the hypothesis that continuous administration of native GnRH, beginning in either early February or March, would induce ovulation without reversion to an anovulatory state following treatment withdrawal. Continuous 28-d treatments elevated circulating luteinizing hormone (LH) and stimulated spontaneous ovulation much earlier than controls. However, mares treated only in February ceased ovarian cycles at termination of treatment. In contrast, mares administered GnRH in March continued to exhibit estrous cycles. Thus, we concluded that GnRH treatment must continue through March to ensure continued escape from winter anovulation. We then tested the hypothesis that the Julian day of conception could be accelerated in winter anovulatory mares treated continuously with native GnRH for 56 d beginning on February 1. Indeed, GnRH treatment caused a marked increase in the frequency of pregnancy compared to controls. Data illustrated that continuous administration of native GnRH is a practical and highly efficient option for managing seasonal anovulation. In Aim II, we examined hypothalamic distribution, adenohyphyseal receptor gene expression, and ligand functionality of RFRP3 in the mare during the breeding and non-breeding seasons. Hypothalamic RFRP3 mRNA was detected in the mare; however, neither hypothalamic expression of RFRP3 nor its anterior pituitary receptor differed between reproductive states. We then used equine adenohypophyseal cell culture to test the hypothesis that RFRP3 reduces the responsiveness of the equine gonadotrope to GnRH. Addition of RFRP3 to cell culture failed to counter the effects of GnRH. Finally, the effects of a RFRP3 receptor-signaling antagonist (RF9) were examined in winter anovulatory mares. A robust increase in circulating concentrations of LH relative to controls was observed in response to RF9 treatments, but treatments had no effect on adenohypophyseal responsiveness to GnRH. Data provide indirect evidence that antagonism of the RFRP3 system by RF9 may be at the GnRH neuronal level.Item National Beef Quality Audit - 2011: Survey of Instrument Grading Assessments of Beef Carcass Characteristics(2012-11-16) Gray, Gatlan 1989-The instrument grading assessments for the NBQA-2011 evaluated seasonal trends of beef carcass quality and yield attributes over the course of the year. One week of instrument grading data?HCW, gender, USDA QG, and YG factors?were collected every other month (n = 2,427,074 carcasses) over a 13-month period (November 2010 through November 2011) from four beef processing corporations, encompassing 17 federally inspected beef processing facilities, to create an overview of carcass quality and yield attributes and trends from carcasses representing approximately 8.5% of the U.S. fed steer and heifer population. Mean yield traits were: YG (2.86), HCW (371.3 kg), FT (1.19 cm.), and LM area (88.39 cm^2). The YG distribution was YG 1 (15.7%), YG 2 (41.0%), YG 3 (33.8%), YG 4 (8.5%), and YG 5 (0.9%). Distribution of HCW was <272.2 kg (1.6%), 272.2 kg to 453.6 kg (95.1%), ?453.6 kg (3.3%). Monthly HCW means were: November 2010 (381.3 kg), January 2011 (375.9 kg), March 2011 (366.2 kg), May 2011 (357.9 kg), July 2011 (372.54 kg), September 2011 (376.1 kg), and November 2011 (373.5 kg). The mean FT for each month was November 2010 (1.30 cm), January 2011 (1.22 cm), March 2011 (1.17 cm), May 2011 (1.12 cm), July 2011 (1.19 cm), September 2011 (1.22 cm), and November 2011 (1.22 cm). The mean marbling score was Small49. USDA QG distribution was Prime (2.7%), Top Choice (22.9%), Commodity Choice (38.6%), and Select (31.5%). Interestingly, from November to May, seasonal decreases (P < 0.001) in HCW and FT were accompanied by increases (P < 0.001) in marbling. These data present the opportunity to further investigate the entire array of factors that determine the value of beef. Datasets utilizing the online collection of electronic data will likely be more commonly used when evaluating the U.S. fed steer and heifer population in future studies. These data indicate the wide array of carcasses produced by the beef cattle industry, and how the frequency of both YG and QG traits change from month-to-month.Item Seasonality and Environmental Heterogeneity during a Late Carboniferous Highstand: Brachiopod Shell Geochemical Records from the Pangean Tropics(2014-12-10) Roark, AndrewThe relationship between Permo-Carboniferous glacial cycles and low-latitude climate remains a subject of vigorous debate. This study investigated seasonality and regional environmental variability in a portion of central equatorial Pangea during a late Pennsylvanian highstand using stable isotope and trace element analyses of brachiopod shells from the Virgilian Ames Member of the Conemaugh Group in the Appalachian Basin, U.S.A. Well-preserved, thick-shelled Neospirifer dunbari specimens were serially sampled across growth bands to elucidate a record of seasonal variability during the life of the organisms. Because Neospirifer only colonized this marginal basin during near-maximum highstands when stable marine salinities were established, these data are a direct proxy for intra-annual climate fluctuations during interglacial times. Additionally, well-preserved specimens of the thinner-shelled brachiopod Crurithyris planoconvexa, which has a wider spatial and stratigraphic distribution within the basin, were analyzed individually. Neospirifer specimens show remarkably little internal chemical variability, with ?^18O generally fluctuating by 0.4? or less and ?^13C by less than 1.5? within a single specimen. Moreover, total variability between all specimens is only ~1.5?. This lack of variation reflects a homogenous, nonseasonal to weakly seasonal climate during the Ames highstand. Both ?^18O and ?^13C are ~1.5 lower than those of other Virgilian specimens from regions with a more proximal connection to the open ocean, suggesting relatively high freshwater influence in the Appalachian Basin during this time, although salinities remained close to marine levels. Thus, brachiopod seasonal records indicate normal moist tropical conditions during this penultimate Carboniferous interglacial, with no evidence for strong monsoonal variations in temperature or rainfall. Crurithyris specimens show similarly homogenous isotopic values that are slightly depleted relative to more nearly marine specimens. There is a weak gradient towards increasing ?^18O and ?^13C in Crurithyris specimens to the west, consistent with decreasing influence of Appalachian runoff waters in that direction. Crurithyris from two sites show anomalously enriched isotopic values, potentially indicating rare, sporadic periods of net evaporation. However, because of Crurithyris? wider temporal distribution, these events may not have occurred during the highstand.Item Temporal Variation in Space and Resource Use of Macaws in the Southeastern Peruvian Amazon(2012-07-16) Adamek, Krista AnneSpace use and resource use of three species of macaws (Ara ararauna, A. chloropterus, and A. macao) were studied for a period of three years in the southeastern Peruvian Amazon. Basic information on wild macaw populations is lacking due to the logistical and behavioral challenges of working with these species in dense rainforest. Population declines world-wide have been attributed significantly to a reduction in food and nesting resources due to habitat loss. This research aims to obtain baseline data on macaws in a region with relatively intact rainforest. Specific objectives were to (1) quantify space use, describe the spatial and temporal variation in movement patterns, explore habitat selection and spatial pattern of resources during the non-breeding season, and (2) identify key nesting and foraging species and determine whether there is seasonal variation in diet, and explore how resources may be related to movements and competition. Individuals from each species were radio-tagged and monitored from 2004 to 2008 by ground, platform, and aerial tracking. Seasonal ranges were estimated using MCP and KDE methods. Diversity and niche measurements and selection were calculated for dietary items, nesting substrate, and habitat. The relationship between palm habitat distribution and A. ararauna movements was explored using landscape analysis techniques. All species had similar home range sizes during the breeding season, ranging from a mean of 1,540 ha to 2,541 ha. Non-breeding ranges were significantly larger for A. ararauna (117,849 ha). Greater than 200 species of plants were consumed, yet seasonal preferences vary. The increase in dietary breadth and decrease in overlap during dry season is unlikely related to food scarcity or competition. Key nesting and dietary species include Mauritia flexuosa, Dipterix micrantha, and Bertholletia excelsa. Palm habitat is a key resource for Ara ararauna and associated with long-distance movements. This research addresses a critical gap in our knowledge regarding macaw movements and resource use in Amazonian rainforest. Despite their mobility, their low fecundity and specialized nesting requirements can impact reproductive success and population growth if habitat loss continues on its current trajectory.