Browsing by Subject "quantitative trait loci"
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Item Phenotypic and Molecular Genetic Analysis of Reproductive Stage Heat Tolerance in Wheat (Triticum aestivum)(2011-08-08) Mason, Richard EstenHeat stress adversely affects wheat production in many regions of the world and is particularly detrimental during reproductive development. The objective of this study was to identify quantitative trait loci (QTL) associated with improved heat tolerance in hexaploid bread wheat (Triticum aestivum). To accomplish this objective, an analysis of both the phenotypic and genetic responses of two recombinant inbred line (RIL) populations was conducted. RIL populations Halberd x Cutter and Halberd x Karl 92 (H/K) both derive heat tolerance from Halberd and segregate in their response to heat stress. A heat susceptibility index (HSI) was calculated from the reduction of three yield components; kernel number, kernel weight, and single kernel weight, following a three-day 38 degrees C heat stress treatment during early grain-filling. The HSI, as well as temperature depression of the main spike and flag leaf were used as measurements of heat tolerance. Genetic linkage maps were constructed for both populations and were used in combination with phenotypic data and statistical software to detect QTL for heat tolerance. In a comparison across the two across populations, seven common QTL regions were identified for HSI, located on chromosomes 1B, 3B, 4A, 5A, 5B, and 6D. Subsequent analysis of temperature depression in the H/K population identified seven QTL that co-localized for both cooler organ temperature and improved HSI. Four of the beneficial alleles at these loci were contributed Halberd. The genetic effect of combining QTL, including QHkw.tam-1B, QHkwm.tam-5A.1, and QHskm.tam-6D showed the potential benefit of selection for multiple heat tolerant alleles simultaneously. Analysis of the H/K population in the field under abiotic stress detected QTL on chromosome 3B and 5A, which were in agreement with results from the greenhouse study. The locus QYld.tam-3B was pleiotropic for both temperature depression and HSI in both experiments and was associated with higher biomass and yield under field conditions. The results presented here represent a comprehensive analysis of both the phenotypic response of wheat to high temperature stress and the genetic loci associated with improved heat tolerance and will be valuable for future understanding and improvement of heat stress tolerance in wheat.Item Quantitative trait loci analysis to identify modifiers genes of the gene opaque2 in maize endosperm(2009-05-15) Gutierrez Rojas, Libardo AndresThe protein quality of maize can be improved by replacing normal Opaque2 alleles with non-functional recessive alleles opaque2 (o2). The allele o2 produces a severe phenotype with soft endosperm enhancing its protein quality but decreasing its agronomical value. Plant breeders have restored a desirable ratio of hard to soft endosperm in o2 germplasm known as Quality Protein Maize (QPM). Neither the mechanism nor the genetic components by which the modification of the endosperm in QPM lines occurs are well understood. To increase the understanding of the genetics of endosperm modification, a population of 146 recombinant inbred lines derived from a cross between the o2 inbred line B73o2 and the QPM inbred line CML161 was evaluated in two Texas locations from 2004 to 2006. Four traits related to endosperm texture were measured and showed significant effect of the inbred lines, high heritability estimates and high genetic correlations. Relative content of the essential amino acids lysine, tryptophan and methionine were measured and showed significant effects of the lines and considerable high genetic correlations and heritabilities. Negative correlation was observed between endosperm texture traits and amino acid content. Quantitative trait loci (QTL) were mapped for traits related to the modification of endosperm texture and the content of lysine, tryptophan and methionine. QTLs clusters for endosperm texture traits were detected on chromosomes 3, 5, 6 and 8 explaining 62-68% of the variation. QTLs clusters for amino acid contents were located on chromosomes 7 and 8 that explained up to 39% of the observed variation. The product of the O2 gene is a transcription factor that affects the expression of a number of endosperm genes. A group of 29 endosperm genes associated with the O2 activity were evaluated in developing endosperm of the recombinant inbred lines. Genomic regions controlling gene transcript abundance in developing endosperm were identified by expression QTL mapping. Evidence is presented of QTL hot spots that segregate in association with endosperm texture modification or amino acid contents and are associated with the regulation of the expression of a group of endosperm genes.