Browsing by Subject "potato"
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Item Effects of Bioactive Compounds from Different Potato Genotypes on Prostate Cancer Development in Athymic Nude Mice(2012-07-16) Turner, SarahPhytochemicals are widely noted for their role in chemoprevention. Potato (Solanum tuberosum L.) is the third most important food crop worldwide and is considered a significant source of antioxidants, providing an ideal delivery system for beneficial compounds. The anti-proliferative and pro-apoptotic properties of potato bioactive compounds have been reported in vitro on human prostate cancer cell lines. However, in vivo studies are limited, and more information is needed to determine the chemopreventive properties of potato in the diet. The objective of this study was to evaluate the effects of potato bioactives on prostate cancer in vivo using a mouse model. Athymic nude mice received xenografts of human prostate cancer cells (PC-3) and were administered extracts of potato bioactives from either the white flesh Solanum bulbocastanum (PI243510) or CO112F2-2P/P (purple-flesh Colorado selection), while control mice received water. Neither potato extract provided a significant reduction in tumor growth nor reduced levels of the pro-angiogenic protein VEGF, but the S. bulbocastanum extract reduced expression of metastasis associated protein 1 (MTA1) in tumors, and both potato extracts reduced MTA1 expression in lungs, suggesting the need for further research on the potential chemopreventive or chemotherapeutic properties of potato bioactives.Item Modeling and characterization of potato quality by active thermography(2009-05-15) Sun, Chih-ChenThis research focuses on characterizing a potato with extra sugar content and identifying the location and depth of the extra sugar content using the active thermography imaging technique. The extra sugar content of the potato is an important problem for potato growers and potato chip manufacturers. Extra sugar content could result in diseases or wounds in the potato tuber. In general, potato tubers with low sugar content are considered as having a higher quality. The inspection system and general methodologies characterizing extra sugar content will be presented in this study. The average heating rate obtained from the thermal image analysis is the major factor in characterization procedures. Using information on the average heating rate, the probability of achieving a potato with extra sugar content may be predicted using the logistic regression model. In addition, neural networks are also used to identify the potato with extra sugar contents. The correct rate for identifying a potato with extra sugar content in it can reach 85%. The location of extra sugar content can also be found using the logistic regression model. Results show the overall correct rate predicting the extra sugar content location with a resolution of 20 by 20 pixels is 91%. In predicting the extra sugar content depth, amounts exceeds 2/3 inches are not detectable by analyzing thermal images. The depth of extra sugar content can be discriminated in 0.3 inch increments with a high rate of accuracy (87.5%).Item Screening potato genotypes for antioxidant activity, identification of the responsible compounds, and differentiating Russet Norkotah strains using AFLP and microsatellite marker analysis(Texas A&M University, 2005-02-17) Hale, Anna LouiseTotal antioxidant activity and total carotenoid levels were evaluated for more than 100 common potato (Solanum tuberosum, L.) cultivars grown in the United States, advanced breeding lines from several Western U.S. breeding programs, and 47 related, tuber-bearing species. An initial assessment of variability for antioxidant activity provided baseline information to be used for potential potato promotion and for the development of new varieties with greater human health benefits. Wide variability in antioxidant levels provided evidence of genetic control of this trait, indicating that it could be possible to breed for enhanced levels of antioxidant compounds in potato. Accessions, varieties, and advanced breeding lines identified in the broad screen as having high antioxidant activity and high total carotenoid levels, were fine screened via HPLC to determine specific phenolic and carotenoid compounds present in potato. The objective of the study was to identify parents for use in the Texas breeding program to develop potato varieties containing increased levels antioxidant compounds. In the broad screen for total antioxidant activity, the 47 related, tuber-bearing species showed a wider range of variability than the cultivated varieties and breeding lines. Based on the DPPH assay, antioxidant activity ranged from 103-648 uM trolox equivalents in the cultivated varieties and advanced breeding lines, while that of the wild species was 42-892. HPLC analysis revealed that the phenolic content of the species, and their cultivated counterparts, was primarily composed of caffeic and chlorogenic acids. Other phenolics identified were p-coumaric acid, rutin hydrate, vanillic acid, epicatechin, t-cinnamic acid, gallic acid, and salicylic acid. The highest phenolic content discovered in the accessions was five-fold higher than the highest of the cultivated genotypes. Carotenoid analysis revealed lutein in the accessions, but the yellow-flesh breeding lines were much higher in carotenoids. In addition to the work conducted on antioxidants, an attempt was made to separate intraclonal variants of the potato cultivar Russet Norkotah. Eleven microsatellite primers and 112 AFLP primer combinations failed to produce any reproducible polymorphisms. The inability to detect differences between the clones could be due to the tetraploid nature of the clones or epigenetic differences not detected by the procedures utilized in this study.