Browsing by Subject "orientation"
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Item Integrated Experimental and Theoretical Approaches toward Understanding Strain-Induced Cytoskeletal Remodeling and Mechanotransduction(2012-10-19) Hsu, Hui-JuActin stress fibers (SFs) are mechanosensitive structural elements that respond to applied strain to regulate cell morphology, signal transduction, and cell function. The purpose of this dissertation is to elucidate the effects of mechanical stretch on cell mechanobiology via the following three aims. First, a sarcomeric model of SFs was developed to describe the role of actomyosin crossbridge cycling in SF tension regulation and reorientation in response to various modes of stretch. Using model parameters extracted from literature, this model described the dependence of cyclic stretch-induced SF alignment on a two-dimensional (2-D) surface on positive perturbations in SF tension caused by the rate of lengthening, which was consistent with experimental findings. Second, the sarcomeric model was used to predict how stretch-induced pro-inflammatory mechanotransduction depends on the mode of strain application. Together with experimental data, the results indicated that stretch-induced stress fiber alignment, MAPK activations and downstream pro-inflammatory gene expressions are dependent on SF strain rate (and related changes in SF tension) rather than SF turnover. Third, to produce biocompatible materials that are both mechanically resilient under (physiological) load and also mechanosensitive, a novel hybrid engineered tissue was developed that transmits strain stimuli to cells residing in three-dimensional (3-D) collagen microspheres. However, the macroscopic stress is largely borne by a more resilient acellular polyethylene glycol diacrylate (PEGDA) hydrogel supporting the microspheres. Careful analysis indicated that cell alignment occurs prior to significant collagen fibril alignment.Item Quantitative characterization of polymer scratch behavior using a standardized scratch test(Texas A&M University, 2007-09-17) Browning, Robert LeeThe lack of a widely-accepted quantitative methodology for evaluating the scratch behavior of polymeric materials has resulted in the development and establishment of a new methodology recently standardized as ASTM D7027-05. Using a custom-built instrumented scratch machine, it is possible to produce controlled, repeatable scratches on polymer surfaces under constant or linearly increasing loading conditions at constant or increasing scratch rates. Software-aided digital image analysis along with material science tools (SEM, OM, FTIR, etc.) allows polymer scratch behavior to be analyzed without the ambiguity inherent in the past. The current work will serve to describe the motivation for the development of this methodology as well as illustrate the effectiveness of the increasing load/constant rate test mode in three case studies. First, it will be shown that an acrylic coating on a steel system exhibits three zones of scratch damage: adhesive delamination, transverse cracking and finally buckling failure. It will be discussed how increases in ductility and thickness serve to improve the scratch resistantance of this coating/substrate system. Improvements in the scratch behavior of thermoplastic olefins (TPOs) through the use of surface-treated talc fillers and the slip agent erucamide will be shown in the second case. It was found that the surface-treatment of the talc likely allows for enhanced migration of the erucamide to the TPO surface, thus lowering the surface friction and greatly increasing scratch resistance. Finally, the effects of processing conditions, namely injection molding, on the scratch behavior of neat i-polypropylene will be represented by the results of scratch tests conducted where the scratch direction was oriented both along and transverse to the polymer melt flow direction. Based on the findings of the study, there appears to be a high degree of surface anisotropy introduced to injection-molded polymers due to complex fluid flow regimes as well as non-uniform cooling properties.