Browsing by Subject "optical"
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Item Development of the Ultrashort Pulse Nonlinear Optical Microscopy Spectral Imaging System(2012-10-19) Lee, Anthony Chien-derNonlinear Optical Microscopy (NLOM) has been shown to be a valuable tool for noninvasive imaging of complex biological systems. An effective approach for multicolor molecular microscopy is simultaneous excitation of multiple fluorophores by broadband sub-10-fs pulses. This dissertation will discuss the development of two spectral imaging systems using the principles of nonlinear optical microscopy for pixel-by-pixel spectral segmentation of multiple fluorescent spectra. The first spectral system is reliant on a fiber-optic cable to transmit fluorescent signal to a spectrometer, while the second is based on a spectrometer with an aberration-corrected concave grating that is directly coupled to the microscope. A photon-counting, 16-channel multianode photomultiplier tube (PMT) is used for both systems. Custom software developed in LabVIEW controls multiple counter cards as well as a field-programmable gate array (FPGA) for 1 Hz acquisition of 256x256x16 spectral images. Biological specimen consisting of multicolor endothelial cells and zebrafish will be used for experimental verification. Results indicate successful spectral segmentation of multiple fluorophores with a decrease in signal-to-noise ratio in the FPGA-based imaging system.Item Fabrication of Annealed Proton-Exchanged Waveguides for Vertical Integration(2011-08-08) Webb, Jacob DouglasThere is a drive for improving the surface uniformity of optical waveguide devices in the photonics lab. This report focuses on the exploration of annealed proton exchange (APE) waveguide fabrication on lithium niobate crystal as a method of producing optical waveguides. These waveguides aim to have little variation in step height or surface roughness in the transition area from the waveguide location to that of the bulk crystal, providing a uniform surface amenable to vertical device integration. This is a substantial improvement over the titanium diffused waveguide process, which can have surface variations in excess of 100nm. It is anticipated that the smoother surface will enable light to couple more easily into photonic devices, such as ring resonators, as compared to the current Ti diffused waveguide process. This work explores the design and fabrication aspects of annealed proton exchange waveguides. A review of literature on modeling hydrogen diffusion into lithium niobate is presented, as well as computer models for simulating the bidimensional fractional hydrogen proton concentration distribution. This is used to determine the change in refractive index of the waveguide needed to simulate the mode propagation and profile in the device. Fabrication processes involved in proton exchange waveguide formation are outlined, and measurements for working devices are presented. Best case loss for current devices are 0.5 dB/cm. These samples exhibit smooth surfaces with only ?60A in variation of surface uniformity. Concluding remarks present ideas to further the work by lowering propagation losses, improving mode matching to single mode fiber, and improving the consistency of fabrication conditions.Item Fast amplitude and delay measurement for characterization of optical devices(Texas A&M University, 2006-10-30) Thompson, Michael ThomasA fast measurement technique based on the modulation phase-shift technique is developed to measure the wavelength-dependent magnitude and phase responses of optical devices. The measured phase response is in the form of group delay, which is used to determine the chromatic dispersion in the device under test by taking the derivative of the group delay with respect to optical wavelength. The measurement setup allows both step-tunable and sweeping laser sources. A modulation frequency of up to 2.7 GHz is accommodated. An alternate method for the phase measurement that overcomes non-linearities in the measurement setup is also presented. The speed of the measurement setup is limited by the sweeping speed of the laser source, which for the Agilent 81682A is 40 nm/sec. The magnitude accuracy is determined by taking a comparison to the commercially available Micron Finisar measurement system, where an error of 0.125 dB is noted. The phase accuracy of the measurement setup is tested by taking the Hilbert transform of the measured magnitude response of an Acetylene gas cell and comparing it to the integral of the measured group delay. The average deviation between the two methods is 0.1 radians. An Acetylene gas cell, fiber Bragg grating, and chirped Bragg grating are tested with the measurement setup and the Agilent 8168The characterization of the setup leads to the conclusion that the measurement setup developed in this paper is fast and accurate. The speed of the technique is on the order of microseconds for a single measurement and excels beyond the speed of the standard modulation phase-shift technique, which includes measurement times on the order of minutes. The accuracy of the technique is within 0.125 dB for magnitude measurements and 0.1 radians for phase measurements when compared to commercially available measurement systems.2A laser source at 40 nm/sec and the measurement plots are presented.Item Laboratory investigation of chemical and physical properties of soot-containing aerosols(Texas A&M University, 2006-08-16) Zhang, DanSoot particles released from fossil fuel combustion and biomass burning have a large impact on the regional/global climate by altering the atmospheric radiative properties and by serving as cloud condensation nuclei (CCN). However, the exact forcing is affected by the mixing of soot with other aerosol constituents, such as sulfuric acid. In this work, experimental studies have been carried out focusing on three integral parts: (1) heterogeneous uptake of sulfuric acid on soot; (2) hygroscopic growth of H2SO4-coated soot aerosols; (3) effect of H2SO4 coating on scattering and extinction properties of soot particles. A low-pressure laminar-flow reactor, coupled to ion driftchemical ionization mass spectrometry (ID-CIMS) detection, is used to study uptake coefficients of H2SO4 on combustion soot. The results suggest that uptake of H2SO4 takes place efficiently on soot particles, representing an important route to convert hydrophobic soot to hydrophilic aerosols. A tandem differential mobility analyzing (TDMA) system is employed to determine the hygroscopicity of freshly generated soot in the presence of H2SO4 coating. It is found that fresh soot particles are highly hydrophobic, while coating of H2SO4 significantly facilitates water uptake on soot even at sub-saturation relative humidities. The results indicate that aged soot particles in the atmosphere can potentially be an efficient source of CCN. Scattering and extinction coefficient measurements of the soot-H2SO4 mixed particles are conducted using a threewavelength Nephelometer and a multi-path extinction cell. Coating of H2SO4 is found to increase the single scattering albedo (SSA) of soot particles which has impact on the aerosol direct radiative effect. Other laboratory techniques such as transmission electron microscopy (TEM) and Fourier transform infrared spectrometry (FTIR) are utilized to examine the morphology and chemical composition of the soot-H2SO4 particles. This work provides critical information concerning the heterogeneous interaction of soot and sulfuric acid, and how their mixing affects the hygroscopic and optical properties of soot. The results will improve our ability to model and assess the soot direct and indirect forcing and hence enhance our understanding of the impact of anthropogenic activities on the climate.Item Multi-layer diffusion approximation for photon transport in biological tissue(2009-06-02) Hollmann, JosephA method for improving the accuracy of the optical diffusion theory for a multi-layer scattering medium is presented. An infinitesimally narrow incident light beam is replaced by multiple isotropic point sources of different strengths that are placed in the scattering medium along the incident beam. The multiple sources are then used to develop a multi-layer diffusion theory. Diffuse reflectance is then computed using the multi-layer diffusion theory and compared with accurate data computed by the Monte Carlo method. This multi-source method is found to be significantly more accurate than the previous single-source method. The appendix to this thesis also shows the derivation of the extrapolated boundary condition. This boundary condition is utilized to solve for the discontinuity that occurs at the tissue-ambient medium interface. The boundary conditions for common index of refraction mismatches are solved for and listed in a table.Item Optical control of nuclear resonant absorption: theory and experiment(Texas A&M University, 2004-09-30) Kolesov, Roman L.Modification of nuclear resonant absorption by means of laser radiation is analyzed both theoretically and experimentally. Theoretical analysis is done on the basis of four-level model of atom. This model includes both electronic and nuclear excitations. It is predicted that under coherent laser driving nuclear resonant Mossbauer absorption can be significantly modified, e.g. new Mossbauer resonances can appear, some of the existing resonances can vanish, both can be Rabi-split, broadened by laser action. In addition, it is predicted that Mossbauer absorption can be completely suppressed due to coherent population trapping. Experimental observation of laser-induced transformations of Mossbauer spectra of 57Fe2+ : MgO is accomplished. New Mossbauer lines appear with laser driving while the existing are broadened. Possible explanations of the observed changes in 57Fe2+ : MgO Mossbauer spectra are population of higher-lying electronic states of iron ion and significant modification of electronic relaxation processes due to modified Jahn-Teller interaction.Item Optical Perfusion and Oxygenation Characterization in a Liver Phantom(2012-02-14) King, Travis J.Continuous monitoring of blood perfusion and oxygenation is essential in assessing the health of a transplanted organ. Particularly, monitoring the perfusion and oxygenation of the organ during the two-week period after the transplant procedure is crucial in detecting a sustained loss in perfusion or a reduction in oxygen saturation before these changes render irreversible damage to the organ or patient. Pulse oximetry is a clinically accepted method of monitoring the arterial oxygen saturation of a patient in a non-invasive manner. Pulse oximeters exploit the wavelength-dependent absorption of oxygenated and deoxygenated hemoglobin to measure a patient's arterial oxygen saturation. However, traditional pulse oximeters do not provide perfusion information and produce erroneous oxygen saturation measurements under low perfusion levels. An optical blood perfusion and oxygenation sensor, based on a modified reflectance pulse oximeter, has been developed for in situ monitoring of transplanted organs. To reduce the number of animal experiments, phantoms that mimic the optical and anatomical properties of liver parenchyma have also been developed. In this work, in vitro data was gathered from dye solutions mimicking oxygenated blood that were pumped through single and multi-layer phantoms mimicking liver parenchyma and through a phantom mimicking the portal vein. A portion of the phantom data was compared to data collected from in vivo occlusion studies performed on female swine to assess the ability of the phantoms to mimic the response observed with changes in blood perfusion through liver parenchyma. Both the single layer and multilayer phantoms showed a similar response to changes in perfusion as the in vivo case. With each phantom, the signal increased linearly with increases in perfusion, but the multilayer phantom showed a higher sensitivity (approximately 30% higher) to changes in perfusion than the single layer phantom. This higher sensitivity would provide the ability to measure smaller changes in perfusion and increase the resolution of the sensor. Also, both parenchymal phantoms showed similar trends in the oxygenation studies, with the R value decreasing with increasing oxygenation. While the observations in this research demonstrate the ability to use both phantoms for in vitro experiments, the results show the multilayer phantom is a better option for mimicking perfusion because it displays similar occlusion patterns as the liver parenchyma in vivo, a higher sensitivity to changes in perfusion than the single layer phantom, and it is only slightly more complex in design (contains only two more layers of sinusoids) than the single layer phantom.Item Ultrasound-modulated optical tomography for biomedical applications(Texas A&M University, 2004-11-15) Li, JunI experimentally studied ultrasound-modulated optical tomography, which holds the promise for biomedical diagnosis. I measured the degree of polarization of laser speckles generated by scattered light transmitted through turbid media, investigated three signal-detection schemes for extracting the intensity of the ultrasound-modulated light, carried out experiments to image thick biological-tissue samples, and studied two techniques providing resolution in the cross-sections containing the ultrasonic axis. The study of degree of polarization presented results important for the understanding of polarization phenomena in turbid media. I explored an optical-filtering based signal detection scheme, improved the parallel-lock-in speckle detection scheme and proposed a speckle-contrast detection scheme. With the speckle-contrast detection scheme, I successfully obtained images of biological-tissue samples up to 50 mm thick. Further I studied frequency-swept ultrasound-modulated optical tomography for sub-millimeter resolution imaging, and developed ultrasound-modulated optical computed tomography that was based on a back-projection image reconstruction method and obtained clear images of biological-tissue samples.