Browsing by Subject "degradation"
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Item Bt vs. non-Bt corn (Zea mays L.) hybrids: effect on degradation of corn stover in soil(2010-07-14) Salvatore, Herminia T.A billion tons per year of genetically modified corn residues are soil incorporated having both direct and indirect effects on the belowground environment, soil carbon (C) sequestration, and nutrient cycling. If Bt genetic modification has non-target effects on corn stover structural/non-structural carbohydrate and nitrogen (N) concentrations, then the degradation rate of Bt-corn stover may be different than that of non-Bt isolines, possibly influencing soil C storage and N mineralization. Thus, this research focused primarily on the comparison of C and N mineralization of corn stover in soil as affected by Bt-trait, plant portion, water-availability and HFC-trait; and secondarily on the existence of Bt-related variations in the chemical structure of corn residues that might affect the degradation rate of stover in soil and consequently the soil C and N dynamics. A laboratory experiment was conducted under non-limiting N conditions with stover of Bt/non-Bt isogenic pairs of two varieties, a ?high fermentable corn? (HFC) line harvested at Snook, Texas and a non-HFC corn line harvested at the irrigated field of Snook and the non-irrigated field of College Station, Texas. The stover was partitioned into three plant portions, incorporated into a Weswood soil and incubated during 223 days. Results showed that the differences observed in the degradation in soil of Bt vs. non-Bt corn stover were dependent on environmental conditions (irrigated vs. non-irrigated settings) and hybrid variety (HFC vs. non-HFC hybrid lines). The structural composition of corn plants was affected by the Bt-trait, HFC-trait, irrigation and their interactions. Variations in the biomass fractions of the initial stover of Bt and non-Bt hybrids had minimum to non-impact on soil C and N concentrations measured at the end of the 223-day incubation period. Lignin concentration was affected by a Bt-trait*variety interaction. There were no significant differences in lignin concentration between non-Bt/Bt-corn derived stovers of the non-HFC variety irrespective of irrigation regime but Bt-hybrids of the HFC variety contained more than twice as much lignin as the non-Bt isogenic plants. The effects of higher lignin concentration on C mineralization rate appeared to be offset by an increased lignin degradability inherent in HFC-trait. Overall, results indicated that the cultivation of Bt-modified maize lines is not likely to have significant effects on soil C or N dynamics compared with the cropping of non-Bt hybrids.Item Constitutive modeling for biodegradable polymers for application in endovascular stents(Texas A&M University, 2008-10-10) da Silva Soares, Joao FilipePercutaneous transluminal balloon angioplasty followed by drug-eluting stent implantation has been of great benefit in coronary applications, whereas in peripheral applications, success rates remain low. Analysis of healing patterns in successful deployments shows that six months after implantation the artery has reorganized itself to accommodate the increase in caliber and there is no purpose for the stent to remain, potentially provoking inflammation and foreign body reaction. Thus, a fully biodegradable polymeric stent that fulfills the mission and steps away is of great benefit. Biodegradable polymers have a widespread usage in the biomedical field, such as sutures, scaffolds and implants. Degradation refers to bond scission process that breaks polymeric chains down to oligomers and monomers. Extensive degradation leads to erosion, which is the process of mass loss from the polymer bulk. The prevailing mechanism of biodegradation of aliphatic polyesters (the main class of biodegradable polymers used in biomedical applications) is random scission by passive hydrolysis and results in molecular weight reduction and softening. In order to understand the applicability and efficacy of biodegradable polymers, a two pronged approach involving experiments and theory is necessary. A constitutive model involving degradation and its impact on mechanical properties was developed through an extension of a material which response depends on the history of the motion and on a scalar parameter reflecting the local extent of degradation and depreciates the mechanical properties. A rate equation describing the chain scission process confers characteristics of stress relaxation, creep and hysteresis to the material, arising due to the entropy-producing nature of degradation and markedly different from their viscoelastic counterparts. Several initial and boundary value problems such as inflation and extension of cylinders were solved and the impacts of the constitutive model analyzed. In vitro degradation of poly(L-lactic acid) fibers under tensile load was performed and degradation and reduction in mechanical properties was dependent on the mechanical environment. Mechanical testing of degraded fibers allowed the proper choice of constitutive model and its evolution. Analysis of real stent geometries was made possible with the constitutive model integration into finite element setting and stent deformation patterns in response to pressurization changed dramatically as degradation proceeded.Item Desertification of high latitude ecosystems: conceptual models, time-series analyses and experiments(2009-05-15) Thorsson, JohannEcosystem degradation in Iceland has been severe since man arrived 1100 years ago. Birch woodlands cover has declined from 25% of the land area, to only 1%. The deforestation is considered to be the initial stage in the land degradation process, followed by surface destabilization, and later erosion. The objective of this study was to quantify and evaluate factors that contribute to the early stages of land degradation in Icelandic ecosystems. Specific objectives were to improve our understanding of how livestock grazing might initiate early degradation stages, elucidate field-based landscape metrics useful for characterizing degradation stages, and to determine if landscape metrics obtained from remote sensing data can be used to detect landscape structure changes and identify degraded and at risk rangelands in real time over extensive and remote areas. A State-and-Transition conceptual model was constructed for the experimental area to identify potential key processes in the degradation sequence, and to formalize research questions. Experimental plots were established in five plant community types representing a space-for-time degradation sequence. Birch seedling (Betula pubescens Ehrh.) growth and survival was reduced with repeated clipping treatment applied to simulate browsing, but the amount of decline varied with plant community type. This suggests that continuous grazing may contribute to deforestation, as regeneration will be reduced over time. Intense grazing treatments, simulating both grazing and trampling, increased surface instability and soil loss compared to grazing only or control, suggesting that intense grazing may contribute to surface destabilization and therefore to land degradation. Erosion appeared to be active in the most intense treatments, also within the woodlands. The data indicate that the woodlands may have lower resilience than the other plant communities as treatment effects appeared quicker there. The woodlands may thus be particularly vulnerable to intense grazing. The landscape metrics used to quantify changes in landscape surface properties over a 51 year period yielded inconclusive results, either because of data limitations or because of non-detectable erosion activity. The results do generally support the proposed S&T model for the experimental area. It is concluded that grazing may contribute to woodland decline, and intensify degradation processes.Item Exploring the Effects of Crosslinking on the Intervertebral Disc(2012-12-10) Kirking, BryanCrosslinking soft tissue has become more common in tissue engineering applications, and recent studies have demonstrated that soft tissue mechanical behavior can be directly altered through crosslinking, but increased understanding of how crosslinking affects intervertebral disc mechanical behavior is needed. In vitro testing of bovine disc and motion segments was used to characterize several important aspects of disc behavior in response to crosslinking after both soaking and injection treatment. The first study was a comparison of different crosslinkers to determine the effect on tensile properties of disc tissue. Circumferential specimens were taken from bovine annulus and then soak treated with an optimized crosslinking formulation or sham solution. A non-contacting laser micrometer was used to measure cross sectional area, after which tension testing until failure was performed to determine yield strain, yield stress, ultimate stress, peak modulus, and resilience. The crosslinkers were observed to produce different changes in the properties, with the measured properties generally increasing. The second study used bilateral annular injections to simulate a clinically relevant delivery method. The dose response of the motion segment?s neutral zone stability metrics against injection concentration was mapped. Concentrations of 20 mM and less had no significant effects on the stability metrics. 40mM demonstrated a change in neutral zone stiffness, while at least 80mM was required to significantly affect neutral zone length. Thus, meaningful changes in joint neutral zone stability were demonstrated using clinically relevant injection and chemical formulations. The third study used combinations of biochemical and accelerated mechanical cyclic loading to degrade gelatin and annulus fibrosus specimens with and without genipin treatment. Genipin crosslinking attenuated changes during cyclic loading to specimen geometry and compliance relative to control samples. Full recovery of genipin treated samples appeared to be hampered, at least partially from continued crosslinking during the accelerated testing. The fourth study tested the effect of genipin crosslinking to resist interlamellar shearing of the annulus lamella. Using a recently reported test method that shears adjacent lamella, crosslinked specimens were noted to have significantly higher yield force, peak force, and resilience compared to sham treated controls, supporting the hypothesis that crosslinking would increase the load bearing ability of the interface.Item Finite Element Analyses of a Cyclically Loaded Linear Viscoelastic Biodegradable Stent(2014-12-03) Murphy, Jason KyleBiodegradable polymers have been in use for biomedical applications such as sutures and various implants for many years. In the recent decade, research into the development of biodegradable cardiac stents has expanded. This is due to a need for a stent to perform its job and then be removed from the site of implantation, as up to 20% of all cases require re-intervention after 6 ? 12 months due to in-stent restenosis, as reported in 2010. In this study, the effect of viscoelastic and mechanical degradation behaviors on the performance of cylindrical annuli that mimic stents under cyclic loadings is examined. Two polymers are considered: poly-L-lactic acid (PLLA) and polyoxymethylene (POM). A numerical algorithm for an isotropic, linear, viscoelastic material with inclusion of degradation is developed and incorporated into the finite element software ABAQUS/CAE via a user-defined material subroutine (UMAT). A constant pressure meant to mimic the arterial wall?s resistance to expansion is coupled with a cyclic pressure equivalent to an ideal resting blood pressure. The degradation considered is defined as strain-induced. Loading is applied under two cases. A linearly ramped loading is first studied, followed by a creep-cyclic study. Circumferential stresses and strains, along with the degradation, are presented for both a simplified cylindrical annulus, as well as two typical real-world stent geometries. It is seen that not only do material properties affect deformation, but geometrical properties have a large effect as well. The uniform stresses and strains developed in the cylindrical annulus are far less than the non-uniform stresses and strains observed in the realistic stent geometries. This is due to localized stress and strains at the junctions in the mesh of the realistic geometries, where stress concentrations are a maximum. As predicted by the time-dependent material properties given for each material from previous experimental studies, the stresses, strains, and degradation observed are strongly dependent on the time-dependent material behaviors.Item Influence of Insulin Resistance on Contractile Activity-Induced Anabolic Response of Skeletal Muscle(2011-02-22) Nilsson, Mats I.Although the long-term therapeutic benefits of exercise are indisputable, contractile activity may induce divergent adaptations in insulin-resistant vs. insulin-sensitive skeletal muscle. The purpose of this study was to elucidate if the anabolic response following resistance exercise (RE) is altered in myocellular sub-fractions in the face of insulin resistance. Lean (Fa/?) and obese (fa/fa) Zucker rats were assigned to sedentary and RE groups and engaged in either cage rest or four lower-body RE sessions over an 8-d period. Despite obese Zucker rats having significantly smaller hindlimb muscles when compared to age-matched lean rats, basal 24-h fractional synthesis rates (FSR) of mixed protein pools were near normal in distally located muscle groups (gastrocnemius, plantaris, and soleus) and even augmented in those located more proximally (P<0.05; quadriceps). Although 2 x 2 ANOVA indicated a significant main effect of phenotype on mixed FSR in gastrocnemius and soleus (P < 0.05), phenotypic differences were partially accounted for by an exercise effect in the lean phenotype. Interestingly, obese rats exhibited a significant suppression of myofibrillar FSR compared to their lean counterparts (P<0.05; gastrocnemius), while synthesis rates of mitochondrial and cytosolic proteins were normal (gastrocnemius and quadriceps), suggesting a mechanism whereby translation of specific mRNA pools encoding for metabolic enzymes may be favored over other transcripts (e.g., contractile proteins) to cope with nutrient excess in the insulin-resistant state. Immunoblotting of the cytosolic fraction in gastrocnemius muscle indicated an augmented phosporylation of eIF4EBP1 (+ 9%) and p70s6k (+85%) in obese vs. lean rats, but a more potent baseline inhibition of polypeptide-chain elongation as evidenced by an increased phospho/total ratio of eEF2 (+78%) in the obese phenotype. Resistance exercise did not improve synthesis rates of myofibrillar, cytosolic, or mitochondrial proteins to the same extent in obese vs. lean rats, suggesting a desensitization to contractile-induced anabolic stimuli in the insulin-resistant state. We conclude that insulin resistance has diverse effects on protein metabolism, which may vary between muscle groups depending on fiber type distribution, location along the proximodistal body axis, and myocellular sub-fraction, and may blunt the anabolic response to voluntary resistance exercise.Item Investigation of a HA/PDLGA/Carbon Foam Material System for Orthopedic Fixation Plates Based on Time-Dependent Properties(2010-01-14) Rodriguez, Douglas E.While there is continuing interest in bioresorbable materials for orthopedic fixation devices, the major challenge in utilizing these materials in load-bearing applications is creating materials sufficiently stiff and strong to sustain loads throughout healing while maintaining fracture stability. The primary aim of this study is to quantify the degradation rate of a bioresorbable material system, then use this degradation rate to determine the material response of an orthopedic device made of the same material as healing progresses. The present research focuses on the development and characterization of a material system consisting of carbon foam infiltrated with hydroxyapatite (HA) reinforced poly(D,L-lactide)-co-poly(glycolide) (PDLGA). A processing technique is developed to infiltrate carbon foam with HA/PDLGA and material morphology is investigated. Additionally, short-term rat osteoblast cell studies are undertaken to establish a starting point for material biocompatibility. Degradation experiments are conducted to elicit the time-dependent properties of the material system at the material scale. These properties are then incorporated into computational models of an internal plate attached to a fractured human femur to design and predict the material response to applied physiological loads. Results from this work demonstrate the importance of material dissolution rate as well as material strength when designing internal fixation plates.Item Non-linear load-deflection models for seafloor interaction with steel catenary risers(2009-05-15) Jiao, YaguangThe simulation of seafloor-steel catenary interaction and prediction of riser fatigue life required an accurate characterization of seafloor stiffness as well as realistic description of riser load-deflection (P-y) response. This thesis presents two load-deflection (P-y) models (non-degradating and degradating models) to simulate seafloor-riser interaction. These two models considered the seafloor-riser system in terms of an elastic steel pipe supported on non-linear soil springs with vertical motions. These two models were formulated in terms of a backbone curve describing self-embedment of the riser, bounding curves describing P-y behavior under extremely large deflections, and a series of rules for describing P-y behavior within the bounding loop. The non-degradating P-y model was capable of simulating the riser behavior under very complex loading conditions, including unloading (uplift) and re-loading (downwards) cycles under conditions of partial and full separation of soils and riser. In the non-degradating model, there was a series of model parameters which included three riser properties, two trench geometry parameters and one trench roughness parameter, two backbone curve model parameters, and four bounding loop model parameters. To capture the seafloor stiffness degradation effect due to cyclic loading, a degradating P-y model was also developed. The degradating model proposes three degradation control parameters, which consider the effects of the number of cycles and cyclic unloading-reloading paths. Accumulated deflections serve as a measure of energy dissipation. The degradating model was also made up of three components. The first one was the backbone curve, same as the non-degradating model. The bounding loops define the P-y behavior of extreme loading deflections. The elastic rebound curve and partial separation stage were in the same formation as the non-degradating model. However, for the re-contact and re-loading curve, degradation effects were taken into the calculation. These two models were verified through comparisons with laboratory basin tests. Computer codes were also developed to implement these models for seafloor-riser interaction response.Item Plant-Soil Interactions, Weed Control, and Rice Tolerance as Affected by Saflufenacil(2012-10-19) Camargo, EdinalvoSaflufenacil is a new herbicide for broadleaf weed control. Limited information is available for crop tolerance, weed control and herbicide behavior in the rice environment. Studies were designed to 1 and 2) evaluate rice tolerance and weed control to saflufenacil in combination with clomazone and imazethapyr; 3) evaluate the absorption and translocation of imazethapyr and saflufenacil in weed species 4) assess saflufenacil degradation and persistence in soils; and 5) investigate the use of reference compounds during the determination of pesticide adsorption (Kd). None or minimal rice injury was observed from preemergence (PRE) application of saflufenacil. Intense injury (68%) was noted with combinations of clomazone (505 g ha-1) applied PRE and saflufenacil (50 g ha-1) applied postemergence (POST). Similarly, rice injury up to 83% was observed in earlier evaluations when saflufenacil was applied POST with imazethapyr. However, subsequent evaluations indicated rice recovery from herbicide treatments. Combination of saflufenacil with imazethapyr resulted in hemp sesbania control ? 88% and red rice control of 100%. Rice yield was not adversely altered by the herbicide treatments used in the clomazone and imazethapyr weed control programs. Imazethapyr plus saflufenacil provided a greater uptake (30%) and translocation (35%) of 14C-imazethapyr than imazethapyr alone in the TX4 red rice. Absorption of 14C-saflufenacil ranged from approximately 40 to 60% in hemp sesbania plants. At 12 and 24 hours after treatment a greater percentage of the absorbed saflufenacil was quantified above the treated leaf at the two lower light intensities. Similar trends were observed for basipetal movement of saflufenacil. An accelerated solvent extraction method was developed to extract saflufenacil from soil. Half-life averaged among soils was 59 and 33 days for saturated and field capacity, respectively. Saflufenacil persistence in the environment was 2 to 3 times longer under flooded conditions for most of the studied soils. Adsorption values were affected by soil to solution ratios, particularly when the soil-pesticide interaction resulted in Kd values > than 2 mL g-1. The use of reference compounds during Kd estimation allowed for calculation of a conceptual adsorption window generating a more comprehensive set of data with alternatives for comparison of soils and methods.Item Thermomechanical Constitutive Modeling of Viscoelastic Materials undergoing Degradation(2012-07-16) Karra, SatishMaterials like asphalt, asphalt concrete and polyimides that are used in the transportation and aerospace industry show viscoelastic behavior. These materials in the working environment are subject to degradation due to temperature, diffusion of moisture and chemical reactions (for instance, oxidation) and there is need for a good understanding of the various degradation mechanisms. This work focuses on: 1) some topics related to development of viscoelastic fluid models that can be used to predict the response of materials like asphalt, asphalt concrete, and other geomaterials, and 2) developing a framework to model degradation due to the various mechanisms (such as temperature, diffusion of moisture and oxidation) on polyimides that show nonlinear viscoelastic solid-like response. Such a framework can be extended to model similar degradation phenomena in the area of asphalt mechanics and biomechanics. The thermodynamic framework that is used in this work is based on the notion that the 'natural configuration' of a body evolves as the body undergoes a process and the evolution is determined by maximizing the rate of entropy production. The Burgers' fluid model is known to predict the non-linear viscoelastic fluid-like response of asphalt, asphalt concrete and other geomaterials. We first show that different choices for the manner in which the body stores energy and dissipates energy and satisfies the requirement of maximization of the rate of entropy production that leads to many three dimensional models. All of these models, in one dimension, reduce to the model proposed by Burgers. A thermodynamic framework to develop rate-type models for viscoelastic fluids which do not possess instantaneous elasticity (certain types of asphalt show such a behavior) is developed next. To illustrate the capabilities of such models we make a specific choice for the specific Helmholtz potential and the rate of dissipation and consider the creep and stress relaxation response associated with the model. We then study the effect of degradation and healing due to the diffusion of a fluid on the response of a solid which prior to the diffusion can be described by the generalized neo-Hookean model. We show that a generalized neo-Hookean solid - which behaves like an elastic body (i.e., it does not produce entropy) within a purely mechanical context - creeps and stress relaxes when infused with a fluid and behaves like a body whose material properties are time dependent. A framework is then developed to predict the viscoelastic response of polyimide resins under different temperature conditions. The developed framework is further extended to model the phenomena of swelling due to diffusion of a fluid through a viscoelastic solid using the theory of mixtures. Finally, degradation due to oxidation is incorporated into such a framework by introducing a variable that represents the extent of oxidation. The data from the resulting models are shown to be in good agreement with the experiments for polyimide resins.