Browsing by Subject "Transformation"
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Item Competence induction and transformation-negative mutants in aeromonas(2011-08) Hailu, Tirhas A.; Jeter, Randall M.; Zak, John; Carty, NancyMembers of the genus Aeromonas can be isolated from many environmental niches where bacteria exist, but are ubiquitously found in aquatic environments and can be isolated from rivers, lakes, ponds, estuaries, and groundwater. The presence of Aeromonas in water systems, especially in drinking water, is an issue of concern in public health. One reason that members of genus Aeromonas are a threat to human health is their ability to multiply in drinking water, and this adaptation may be due to their ability to undergo transformation. Transformation is one method of bacterial gene transfer, in which extracellular (“naked”) DNA is taken up from the environment, and it is entirely dependent on the competence of the recipient cell. Competence is the physiological state that the cell must enter before it can take up extracellular DNA. Competence in Aeromonas can be induced under laboratory conditions. The nutrient strength and the content of culture media can affect competence induction in Aeromonas. Competence induction is higher in media which have lower nutrient concentrations than in full-strength media. Several factors, such as the pH of the medium and molecules secreted by a growing culture, putative quorum-sensing molecules, can also have an effect on competence induction in Aeromonas. This research found that, the competence of Aeromonas was highest in the range of pH 6 to pH 8. Media with a pH below 6 or above 9 produce reduced or low numbers of transformants. The putative quorum-sensing molecules negatively affected competence induction in Aeromonas. Four putative transformation-deficient strains were isolated by random insertion mutagenesis of strain C-70 and tested for their ability to transform using qualitative and quantitative transformation assays. There were none or greatly reduced numbers of transformants in all the four strains.Item Design with innovation: Transforming an urban playa stormwater site(2008-12) Glancy, Candi Lu; Klein, Charles H.; Haukos, David A.; Billing, John C.Stormwater management in the Playa Lakes Region of West Texas historically requires that playa wetlands in city limits be modified to collect runoff from seasonal storms. These urban playas often serve a second function, that of an open space park. Design of these parks frequently lacks innovation in aesthetics and stormwater cleansing functions. Creativity in stormwater solutions extends beyond the typical functional-only design. In cities such as Renton, Washington, Minneapolis, Minnesota, and Gilbert, Arizona, stormwater and wastewater have been innovatively incorporated into the urban culture. This thesis explores an innovative solution for the Playa Lakes Region of West Texas that goes beyond what was discovered in the literature review. Design criteria are developed to transform Lake 87, a Lubbock, Texas urban stormwater management site, into an aesthetically pleasing, artistic, innovative site that integrates the history and nature of the area through native plantings and sculpture without compromising its primary function. The design also resolves increasing erosion through innovative best management practices (BMP) and landforming. This thesis investigates playa wetlands (Lake 87’s origin), urban stormwater function and management practices (Lake 87’s role in the community), aesthetic and artistic design solution case studies, and the role of art in society (Lake 87’s potential). The innovative design solution for Lake 87 has a complexity that appeals to the senses so that different interpretations are possible. The design attempts to engage the participant by becoming part of the harmony and contrast that is present in nature. It is an exploration of an alternative in stormwater management.Item Everything is liquid(2014-05) Swan, Taylor; Rifkin, Ned; Higgins, Kathleen MarieIf we are to imagine universal reality--a reality where both subjectivity and objectivity exist--mapped as a hollow sphere, the outer shell that defines its physical presence would be the objective reality and the vacuum of space encased would be subjectivity. The hollow core is not defined by--or representative of--a singular piece of the shell, but only of the shell in its entirety. The human subject solely roams this inner space since they are nothing outside themselves. Their task is to wander the spectrum of subjectivity completely until they have reached objectivity. The objective reality only defines shape and mass. It is concrete and monolithic. Energy is distributed equally along its surface and each point reinforces the structural existence of the adjacent points. All of humanity is a varying degree of subjectivity, but the spectrum is finite with objectivity always at one end; objectivity is subjectivity's ultimate experience. Death is the crystallization into the shell of objectivity for the subject becomes pure matter and form that is severed from will. It seems then the only real limit to an individual’s experience is death, the human's subjective experience permeating into objectivity and transforms from a state that is on the inside perceiving out, to a state that is outside of itself, which is to say nothing. It is possible to observe this transformation as a continuous seam of reality with varying degrees of transformation, and not a bold division of two states.Item The (fe)male shifts shame : androgyny and transformation in Marie de France, Gerald of Wales, and the Volsungasaga(2013-05) Gutierrez-Neal, Paula Christina; Wojciehowski, Hannah Chapelle, 1957-; Blockley, MaryTransformation is inherently entwined with the transgression of borders; for male shifters, there is an acquittance of this transhuman breach, but not so for female shifters. Gerald of Wale's History and Topography of Ireland depicts two werewolves: the male's shapeshifting is all but disregarded, while the female's own transformation is depicted in detail and effectively shames her into silence. In addition, the Volsungasaga also contains werewolves: Sigmund and Sinfjotli don wolfskins, but soon regret their transformations. However, neither is shamed for the shapeshifting, and indeed, Sinfjotli successfully twists the experience to his advantage. The female werewolf, King Siggeir's mother, however, is killed and her identity as a "foul" witch exposed. There are also the human-to-human transformations of Signy/a witch and Sigurd/Gunnar. Signy expresses shame for the incident; Sigurd and Gunnar's plot is revealed, but neither is condemned: the tale passes over the shapeshifting in favor of the narrative drama. Furthermore, Marie de France's Bisclavret perpetuates the same pattern: the male werewolf is praised and exonerated for his transhuman nature while the wife's pseudo-shapeshifting is met with condemnation and shame. However, Marie de France's Yonec attempts to break this pattern, with the shapeshifter Muldumarec transgressing not only the animal/human binary but that of the male/female. His androgyny is conferred onto his beloved, who also undergoes transformations but is spared the shaming consequences via Muldumarec. While this sharing of androgyny breaks the pattern and keeps the beloved from condemnation, it ultimately fails in breaking the patriarchal underpinnings of the pattern itself.Item Humble alchemy(2014-05) White, Shalena Bethany; Williams, Jeff, M.F.A.This master's report addresses the conceptual and material investigations that were explored within my artistic research made at the University of Texas at Austin between 2011 and 2014. These works are a confluence of adornment, sculpture and installation art. These pieces incorporate ancient and contemporary metalworking techniques with raw, organic material. The notion of elegant ornamentation is expanded beyond the body into the adornment of architecture. The potential for transformation and reinvention within found elements is explored within this work. The natural resources I work with have gone through a cycle, which is interrupted when the objects are removed from the earth. I see my process in relationship to alchemical concepts of transmutation. Through manipulation, common matter evolves into precious material. The refined, meticulous craftsmanship conveys a sense of reverence and honor towards the common material. This intervention with the material is an act of preservation and veneration. This work explores my sense of intrigue about the extraordinary potential of mundane materials, and investigates conventional notions of material value.Item Managing academic and personal life in graduate studies : an interactive qualitative analysis of graduate student persistence and transformation(2011-08) Winston, Rachel Anne; Roueche, John E.; Northcutt, Norvell; McClenney, Kay; McCombs, Maxwell; Butler, Jess; Reddick, Richard; McCoy, DannyThis study examines the impact of academic and personal life on graduate student persistence and transformation. Of particular interest are the relationships, emotions, and life management skills required throughout the graduate experience and how socialization, emotional intelligence, and advising aid students through their academic program. With an average of seven to eight years required to complete a doctoral program, life happens. Students enter and leave relationships, children are born, family members have emergencies, health issues arise, and emotional growth takes place. Therefore, students transform not only academically, but in many ways. These are intertwined as evidenced by the data-derived system representation. The importance of understanding the interconnected links in graduate experience spans academic, social, economic, and societal spheres. Each year hundreds of thousands of students enter graduate school. However, for doctoral students, there is an enormous gap between acceptance and completion. After seven years, approximately 50 percent complete their program and after ten years the rate climbs to only 57 percent (Council of Graduate Schools, 2010). This study offers a systemic representation and a four-stage model of graduate student development, incorporating student-identified factors: Faculty Impact, Life Management, Relationships, Playing the Game, Growth/Transformation, Emotions, and Reward/Purpose. Stage I: Orientation and Socialization Stage II: Adjustment and Transition Stage III: Navigation and Transformation Stage IV: Completion and Advancement The results, presented as a systems-based model, along with analysis, may be used to support faculty, advisors, and administrators in creating better advising, orientation, evaluation, and support systems. Departmental policies may be improved to identify at-risk students, provide mentorship opportunities, or obtain continual feedback to understand the underlying factors that may stop students from progressing. This research might also help identify students during the application/admission process. The methodological framework used to create the system produced in this study is Interactive Qualitative Analysis (Northcutt & McCoy, 2004), a methodology that provides the quantitative rigor of algorithmically generated data analysis, combined with the qualitative descriptiveness of interviews, in order to provide insights into the drivers of graduate school persistence. This methodology uses a systematic, protocol-driven research procedure to construct a unified, descriptive diagram to illustrate the phenomenon.Item Rice Transformation as a Means to Study Gene Expression(2010-10-12) Jiang, YimingAn exceptionally effective transformation procedure has been established by using class I embryo-derived rice callus. Every treated callus clump yielded multiple independently transformed plants (average 40 plantlets). Analysis of genomic DNA blots and pollen expressing green fluorescent protein (GFP) from T0 plants revealed that 64% bore a single locus T-DNA insertion in which half had one T-DNA copy. Additive transgene expression was observed fromT0 plants with GFP driven by mUbi1 promoter. Transgenic plants could be rapidly characterized by analyzing GFP pollen from T0 plants without the need for further generations or genomic DNA blot analysis. Agrobacterium tumefaciens-mediated transformation of microspore-derived callus for generating large numbers of T-DNA haploid and doubled haploid(DH) plants has also been investigated. The established transformation procedure resulted in 100% transformation frequency for class I microspore-derived rice callus. Each callus typically yields multiple independent transgenic plants. Genomic DNA blot analysis suggested 98% of the transgenic plants are independent events. About half of the transgenic plants were identified as haploid plants, whereas half are DH hemizygous or homozygous transgenic plants. DH homozygous transgenic plants were obtained from T0plants and confirmed by pollen GFP expression and genomic blot analysis in T0transgenic DH plants. In this study, about 60% ofT0transgenic DH plants had a single locus T-DNA insertion of which 45% bore one T-DNA copy. Furthermore, in a population of over 2,000 haploid and doubled haploid T-DNA plants , about 25% showed phenotypic differences from non-transformed haploid plants. Approximately 5% were seriously phenotypically abnormal including lethal or semi-lethal mutants. This highly efficient transformation procedure using microspore-derived callus could be valuable in speeding up plant breeding and in new gene discovery. Diversification of the mUbi1 promoter led to a minimal promoter that has a similar function as the original mUbi1. Transient and stable transformation as measured from gene expression driven by the minimal promoter suggested that it has a similar function as the original wild type promoter.Item Students from India at a Major Research University in the United States: A Phenomenological Study of Transition, Adjustment, and Transformation(2011-08-08) Chennamsetti, PrashantiThe purpose of this qualitative study was to explore the phenomena of transition, adjustment, and transformation among adult graduate students from India, who made the transition to Nurture University in the southwestern part of the United States. According to Open Doors 2008, since 2001, a majority of the international students in U.S. higher education has been from India. Because of the significant cultural gap between India and the United States, Indian students encounter adjustment difficulties in the host country. To overcome such difficulties, students apply several coping strategies, which in turn lead to a transformational change among them. The transitional adjustment literature on the experiences of the students from India has been very limited. This lack of attention to Indian students, despite the fact that their percentage has been increasing in the U.S. higher education, is an issue of concern and, therefore, calls for research. To achieve this goal, a phenomenological data analysis process presented by Moustakas's modified version of Stevick-Colaizzi-Keen's method was utilized. Data analysis generated the following themes: individual experiences, situational experiences, coping strategies/suggestions, and personal transformation. Under individual experiences, the participants described their experiences in the United States, and differences between their expectations and experiences in relation to Nurture University and general living. Under the situational experiences, study participants described the differences between being a student in the United States versus in India, and the differences between the academic systems of United States and that of India. Further, the application of imaginative variation in step 7 of the data analysis led to the identification of two sub-themes under the theme individual experiences: individual factors that helped in adjustment and individual factors that hindered adjustment in the United States. Sub-themes identified under situational experiences were situational factors that helped in adjustment and situational factors that hindered adjustment in the United States. This study has implications for the home country, host country, adult education, higher education, policy, theory and future research. To ensure the trustworthiness of the data, qualitative procedures such as Epoche, thick descriptions of the phenomenon of transition and member checks, were employed.Item TGF-β/Smad signaling is important for v-Rel mediated transformation(2010-05) Tiwari, Richa; Bose, Henry R.; Tucker, Phillip W.; Dudley, Jaquelin P.; Tian, Ming; Van Den Berg, CarlaThe v-rel oncogene is the most efficiently transforming member of the Rel/NF-κB family of transcription factors. Identification of genes or signal transduction pathways that contribute to v-Rel transformation provide insight into the mechanisms of tumorigenesis by Rel/NF-κB proteins. In these studies, the contribution of TGF-β/Smad signaling to v-Rel transformation was assessed. TGF-β/Smad signaling regulates several cellular processes, including growth, differentiation, and apoptosis and has been implicated in a number of different cancers. Using microarray technology and Northern blot analysis, key components of the TGF-β/Smad pathway (tgf-β2 and tgf-β3 ligands, TGF-β type II receptor, and receptor-activated smad3) were identified with upregulated mRNA expression in v-Rel-transformed fibroblasts and lymphoid cells relative to control cells. A corresponding change in their protein levels was also observed. Further analysis revealed elevated levels of the phosphorylated, active form of Smad3, which correlated with its increased DNA-binding activity in v-Rel transformed cells. In contrast, the overexpression of c-Rel resulted in little to no alteration in the RNA and protein expression of members of the TGF-β/Smad pathway. Further studies demonstrated that elevated TGF-β/Smad signaling is required for the transforming ability of v-Rel. Blocking TGF-β signaling with a kinase inhibitor of TGF-β type I receptor inhibited the activation of Smad3 and dramatically reduced the ability of v-Rel transformed cells to form colonies in soft agar. Overexpression of a constitutively active form of Smad3 in the inhibitor-treated cells restored their ability to form colonies in soft agar close to the levels seen in untreated cells. Additional experiments with dominant negative Smad3 also revealed its ability to hinder the oncogenic potential of v-Rel. In complementary experiments, a stimulatory effect on v-Rel transformation was observed with cells treated with recombinant TGF-β2 ligand or overexpressed with wild-type Smad3. Taken together, these studies demonstrate that TGF-β signaling is crucial for the transformation potential of v-Rel and is primarily mediated by Smad3 activity.Item Transformation from Developmental Mathematics Student to Mathematics Teacher: Narratives of Adult Learning Experiences(2010-01-16) Wright, Gary L.The purpose of this study was to gain a better understanding of the experiences of developmental mathematics students who, after successful completion of their developmental courses, chose a career in teaching and to gain a better understanding of how those experiences helped shape their decision to go forward with a career in mathematics education. With the intention of exploring the text and the context of the experiences of former developmental mathematics students as they have reflected on them and storied them so as to make meaning of them, I determined that a qualitative methodology was indicated; and the qualitative method selected was narrative analysis. Altogether 13 respondents met the criteria and were interviewed. Interviews conducted during the spring and early summer of 2008 were recorded, transcribed, and analyzed for data relevant to the goals of this study. Analysis revealed that developmental mathematics students not only have the capacity to become competent students but they have the potential of becoming outstanding teachers and scholars. This potential is closely tied to affective qualities, such as self-efficacy, which are often profoundly impacted and enhanced by a teacher or mentor. The developmental student who has chosen a career in education frequently views the teacher/mentor as (i) a role model who he/she desires to emulate thereby extending that profound impact, and/or as (ii) a hero for whom future endeavors are viewed as a form of payback. The narratives also revealed that women developmental students typically had greater struggles and difficulties in meeting their educational goals because they bore the responsibility for caring for children and, in most cases, for their family?s financial support. Areas of study that warrant further investigation were uncovered while doing this research and include (i) an identification of teaching methodologies that both enhance mathematics capability and also bring a greater self-awareness of the increased capability, (ii) a determination of the impact of faculty/institutional behaviors and attitudes on adult developmental mathematics students who dropped out of their programs and did not complete their college education, (iii) the characteristics of the educational experiences of single mothers who passed through developmental mathematics and on to the teaching profession, and (iv) a deeper understanding of the teacher recruitment potential of cooperative learning groups, tutoring centers, and supplemental instruction.Item Transformational indicators : deciding when to develop transformable products(2010-08) Camburn, Bradley Adam; Wood, Kristin L.; Jensen, DanTransformable products (or transformers), those with two or more functional states, are increasingly utilized by our society. As the mobility and complexity of life increases, so must the adaptability of the products which we use. We need new design techniques to develop more adaptable devices, such as transformers. The purpose of this study is to propose a response to the question “When is it preferable to implement a design approach focused on developing transformable products over an approach focused on developing primary function, non transforming products?” Our response to this question comes in the form of a method. The method helps a designer or design team consider the benefits of developing a transformer at an early stage in the design process. Research includes a deductive and an inductive study which are used to identify transformation indicators or context properties and usage factors that identify when it is preferable to build a transformable device. Static function-state indicators are also presented. These are contrary to transformation indicators in that they identify contexts suitable for developing non-transforming devices. Our technique seeks to improve the outcome of a design project by encouraging the consideration of transformable solutions and aiding in the selection of an appropriate design process. This method for testing the presence of these indicators in exemplary design contexts is presented. One such application is the design of an autonomous bridge-health monitoring system.