Browsing by Subject "Ovalbumin"
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Item A quantitative study on the denaturation of ovalbumin by shaking(Texas Tech University, 1966-05) Wolfe, Frederick HenryNot availableItem A study of the amino acids associated with ovalbumin(Texas Tech University, 1970-12) Hicks, John EdgarNot availableItem An electrophoretic and spectrophotometric study of the denaturation and coagulation of ovalbumin by shaking(Texas Tech University, 1966-05) Darnall, Dennis WNot availableItem Effect of Collection Method and Archiving Conditions on the Survivability of Vegetative and Spore Forming Bacteria(2010-10-12) Kassab, Asmaa S.To ensure effective detection of bio-particles, it is crucial to understand the effects of collection method and archiving conditions on the survivability of bioaerosols, consequently, the survivability of the spore-forming Bacillus globigii (BG) and MG1655 Escherichia coli (E. coli), was determined after collection. The survivability was defined as the culturable fraction of the archived bacteria/culturable fraction of the as-collected bacteria. The bacteria were aerosolized for up to four days at room temperature (RT, 25 degrees C) and at 4 degrees C and collected in a 100 L/min wetted wall cyclone (WWC) and a 12.5 L/min SKC BioSampler. Aqueous solutions of 0.01% Tween-20 and 30% Ethylene Glycol (EG), with or without 0.5% ovalbumin (OA), were used as the collection fluids. Antifoam B (A-F), at a concentration of 0.2% (V:V) was added to the BG samples containing OA. In general, samples archived at 4 degrees C showed higher survivability than at RT. The survivability were more stable in EG than in Tween-20 especially for BG, very likely due to the surfactant effect of the Tween-20, which would remove the spore coat and initiate germination. In the WWC, adding OA significantly increased the survivability of BG in EG and in Tween-20, especially at RT. Similar effect of OA was found for E. coli samples stored in EG, suggesting that OA might be beneficial in maintaining the survivability. Adding A-F increased the survivability of BG in EG. In the SKC, neither the addition of OA nor A-F seems to have a beneficial effect on the survivability of the spores in EG samples. The best collection fluid for maintaining survivability in the WWC is EG+A-F for BG, and EG+OA for E. coli. However, in the SKC, EG is the best for BG collection and Tween-20 for E. coli. Viability transfer ratios, VTR, (cells surviving collection at time zero/viable cells aerosolized) were calculated for both devices. A performance ratio was calculated as the VTR of the WWC/VTR of the SKC. The geometric mean of the performance ratio is 1.51+/-0.83 for BG and 2.60+/-0.16 for E. coli, indicating that viability transfer ratio of the WWC is typically higher than that of the SKC.Item Stress and allergic pulmonary inflammation(2006-12) Hulbert, Lindsey E.; McGlone, John J.; Shome, Goutam P.; Carroll, Jeffery A.; Vizcarra, Jorge A.The interactions between stress and allergic pulmonary inflammation are not well understood. Experiments were conducted utilizing the ovalbulmin (OVA) sensitization and aerosol challenge to induce allergic pulmonary inflammation in mice and stimulate the T helper (Th2) milieu immunity in female Balb/c mice. An Ovalbumin (OVA) sensitization and aerosol challenge model was utilized. The first study utilized an amphetamine withdrawal model as the stressor. There were no significant immune x stress interactions. An additive effect was observed for some Th2 cytokines and lung inflammation. The second study evaluated lipopolysaccahride challenge and forced swim test (FST), effects on mouse plasma corticocosterone levels at 20, 50, and 80 min after the start of exposure. FST treated mice, 50 minutes after exposure had the highest levels of corticosterone. Control mice also had elevated levels of corticosterone compared to literature baseline levels. The third study attempted to establish a time when full lung inflammation occurred. Sampling times included 0, 12, 24, 36 and 48 h after aerosol challenge. Mice harvested 0 to 24 h post allergen exposure had the most complete inflammatory response (not significant). The final study examined the effects of the FST on allergic pulmonary inflammation using the OVA induced allergy mouse model. Th2 cytokines in BAL fluid were elevated among allergic mice, the stressor reduced IL-5 and IL-6 concentrations in allergic mice, while the stressor alone (non-allergic) caused an increase in IL-4 and IL-10.Item The reaction of N-acetylimidazole with denatured ovalbumin: and the determination of exposed tyrosyl residues(Texas Tech University, 1968-08) Grandjean, Carter JulesNot available