Browsing by Subject "Organogenesis"
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Item Complex eye formation in the squid Doryteuthis pealeii and its evolutionary implications(2015-12) Koenig, Kristen Marie; Gross, Jeffrey Martin; Marcotte, Edward M.; Juenger, Thomas; Zakon, Harold; Stein, DavidPhotoreception is a ubiquitous sensory ability found across the Metazoa, and photoreceptive organs are intricate and diverse in their structure. While the morphology of the compound eye in Drosophila and the single-chambered eye in vertebrates have elaborated independently, the amount of conservation within the “eye” gene regulatory network remains controversial with few taxa studied. To better understand the evolution of photoreceptive organs, we established the cephalopod, Doryteuthis pealeii, as a Lophotrochozoan model for eye development. Utilizing histological, transcriptomic and molecular assays we characterize eye formation in Doryteuthis pealeii. Through lineage tracing and gene expression analyses, we demonstrate that cells expressing Pax and Six genes incorporate into the lens, cornea and iris. Functional assays demonstrate that Notch signaling is required for photoreceptor cell formation and retina organization. This comparative approach places the canon of eye research in traditional models into perspective, highlighting complexity as a result of conserved or convergent mechanisms.Item Transcriptional Regulation of Cardio-pulmonary Development(2004-01-14) Aiyer, Aparna R.; Srivastava, DeepakOrganogenesis is a complex process, disruption of which results in developmental anomalies. In recent years, genetic dissection of the pathways involved in cardiogenesis have shown a striking similarity in molecular mechanisms across species. One conserved protein is dHAND, a basic helix-loop-helix (bHLH) transcription factor that is required for normal development of the right ventricle, the pharyngeal arches and limb buds. Loss of dHAND leads to apoptosis in the aforementioned tissues and to embryonic lethality at E10.0. A differential display analysis was performed to identify genes dysregulated in dHAND-/- hearts.