Browsing by Subject "Essential fatty acids"
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Item Linking fatty acids in the diet and tissues to quality of larval southern flounder (Paralichthys lethostigma)(2014-08) Oberg, Erik Winston; Fuiman, Lee A.Essential fatty acids are necessary for growth, survival, and development of larval fishes, but there is limited information on the essential fatty acid requirements of larval southern flounder (Paralichthys lethostigma). The objectives of this study were to elucidate connections between dietary supply of docosahexaenoic acid (DHA) and arachidonic acid (ARA) and deposited fatty acids in the head or body, and then link diet and stored fatty acids in the head or body with larval quality traits. From 4-15 days posthatch (dph), southern flounder larvae were fed rotifers enriched with four different combinations of DHA-rich Algamac 3050 and ARA-rich Algamac ARA. Fatty acid concentrations in the head and body were measured at 15 dph, and relationships between fatty acids in head or body and in the diet were determined. Larval quality traits, including specific growth rate (SGR), survival, and eight behavioral performance variables were measured. Results showed that concentrations of DHA and ARA in the head and in the body were correlated with concentrations of DHA and ARA in the diet. Growth rate did not vary among the four diets, but survival was positively correlated with the amount of lipid in the diet. Responsiveness to a visual stimulus was positively correlated with the concentration of DHA in the diet, the ratio DHA:EPA in the head, and total energy content of the diet. Turning rate during routine swimming was correlated with body DHA. This study demonstrates the influence of DHA content, total lipid content, and energy levels in the diet of southern flounder and provides a foundation for future studies examining causal factors of recruitment variability or larviculture production success.Item Tracing organic matter pathways in marine food webs using fatty acids and compound specific stable isotope analysis(2015-08) Smith, Stephanie Denise; McClelland, James W.; Dunton, Kenneth H; Walther, Benjamin DOrganic matter inputs to the marine environment vary over seasonal and spatial scales, altering the type and availability of food sources for marine consumers. It is important to identify diet in order to understand basic ecology, characterize trophic interactions, and predict consequences of biotic and abiotic change within a community. Methods of direct observation of diet and feeding can be difficult, so indirect methods have been developed such as analysis of gut contents and fecal pellets. However, these methods only represent a snapshot of the last meal, and provide information about what was ingested, but not what was actually incorporated into consumer tissues. Therefore, biogeochemical approaches such as fatty acid (FA) and stable isotope analyses have been developed, which provide a time-integrated measure of diet. Further, stable isotope measurements of specific FA markers can be used to identify carbon sources, and can be applied to a variety of food web studies (Iverson et al., 2004). The purpose of this research is to examine the linkages between organic carbon sources and trophic transfer by consumers. To achieve this, we use FA biomarkers and compound specific stable isotope analysis (CSIA) to trace carbon cycling. This study has two main components: environmental sampling and experimental research. Chapter 1 demonstrates the use of these tools for elucidating seasonal trophic linkages in invertebrates collected from the Alaskan Arctic coast. Overall, invertebrate diets were characterized by terrestrial, detrital, and carnivorous sources in winter and spring, with a shift toward autochthonous diatom-based diets in summer. Our results demonstrate the importance of terrestrial organic carbon as a subsistence food source in winter, whereas in situ production in summer was critical for accumulating FA stores rich in essential FAs. Chapter 2 is an experimental feeding study designed to quantify the incorporation rates of 18:2n-6 from diet to tissue in Atlantic croaker. Liver tissues accumulated FAs more quickly than muscle tissues, but both tissues reached equilibrium at 5 to 7 weeks. From these experiments, quantitative assessments of diet sources can be made with confidence when using FAs to understand trophic interactions of Atlantic croaker and other similar species.