Browsing by Subject "DNA binding site"
Now showing 1 - 1 of 1
Results Per Page
Sort Options
Item Approaching the crystal structure of the polymerase γ catalytic complex(2011-08) Meng, Qingchao, master of arts in cell and molecular biology; Yin, Yuhui Whitney; Molineux, IanIn this thesis, a 4.7Å crystal structure of the human mitochondria DNA polymerase γ catalytic complex is reported. Though the DNA substrate-binding site is not identifiable in the structure, two conformational changes in the enzyme architecture are described: 1) rotation of the distal monomer of the accessory subunit towards the catalytic subunit, and 2) shift of the thumb motif of the polymerase domain towards the active site. Both conformational changes suggest a structure of Pol γ in the DNA-bound state and in its active site “closed” conformation.