Browsing by Subject "Colorectal cancer"
Now showing 1 - 3 of 3
Results Per Page
Sort Options
Item Living with colorectal cancer : naturalistic assessment of daily life(2010-08) Rooney, Stephanie Buell; Rude, Stephanie Sandra; Taylor, Cindy C.; Drum, David; Langer, Shelby; McCarthy, Christopher; Pennebaker, JamesEcological momentary assessment provides a unique way of studying quality of life factors of colorectal cancer patients. It has yet to be used to study the behavioral expression of distress or depression by colorectal cancer patients. The current study utilized the Electronically Activated Recorder (EAR) technology to capture the daily activities and conversations of forty-eight adults with colorectal cancer. The study had two purposes: 1) to test the feasibility of the EAR with colorectal cancer patients; 2) to examine separate (self-report and behavioral) indicators of physical functioning, coping, and social support for their relationship to depression. Study participants wore the EAR, a portable digital recorder, for two consecutive days as the EAR recorded 30 seconds every 12.5 minutes. The EAR digital data were transcribed and analyzed for behavioral and linguistic indicators of physical functioning, coping, and social support. The acoustic data were analyzed using the standardized coding system Social Environment Coding of Sound Inventory (SECSI) and the Linguistic Inquiry and Word Count (LIWC2007) computer program. The results provided preliminary evidence that the EAR operated as a feasible and non-disruptive tool for gathering naturalistic data about colorectal patients’ lives. The EAR data revealed information about both the colorectal patients’ internal emotional world as well as their external world which was characterized by solitary acts of daily living. Study subjects were more likely to accept and receive tangible support from others than directly discuss their cancer with others. Analysis of language found that personal disclosure to others was associated with coping through emotional support while causation words (e.g., because, effect, hence) were significantly related with self-report cognitive scales. Furthermore, the study found that first-person singular pronouns were associated not only with depression, but with appraisal of social support. Lastly, a predictive model was tested to see whether self-reported tangible and emotional support and behavioral coding of emotional support each contributed uniquely to the prediction of depression. Only self-reported tangible support was found to significantly predict depression.Item Pathological role of the RON receptor tyrosine kinase in colorectal cancer: Characterization of a short-form RON variant(2006-08) Spidel, Celee M.; Wang, Ming-Hai; Weidanz, Jon; Srivenugopal, Kalkunte; Gunaje, Jayarama; Abbruscato, Thomas J.RON is a member of the MET family of receptor tyrosine kinases. When activated by its ligand, macrophage stimulating protein (MSP), RON initiates downstream signaling events that result in cell dissociation, migration, and invasion. Several RON variants that arise through mRNA splicing or by an alternate translational start site have been identified, some with oncogenic potential. The purpose of the present study was to characterize and determine any pathogenic roles of a naturally occurring 52 kD N-terminal truncated RON (sf-RON). Sf-RON was found to be overexpressed in 37% of 35 primary and 30% of 10 metastatic colorectal carcinomas (CRCs). Sf-RON was also expressed and constitutively phosphorylated in three CRC cell lines. Cloning sf-RON from a CRC cell line and transfecting it into AA/C1 colon epithelial cells resulted in constitutive activation with increased Map kinase p44/42 (ERK1/2) phosphorylation. Sf-RON expression also caused a decrease in E-cadherin, an important cell adhesion molecule whose downregulation is associated with poor survival in cancer. These changes in cell signaling were accompanied by a change in cell-shape and increased cellular growth and migration. Taken together, sf-RON may play a role in the progression of CRC to a more malignant phenotype.Item Pathological role of the RON receptor tyrosine kinase in colorectal cancer: characterization of a short-form RON variant(Texas Tech University, 2006-08) Spidel, Celee M.; Wang, Ming-Hai; Gunaje, Jayarama; Weidanz, Jon; Srivenugopal, Kalkunte; Abbruscato, Thomas J.RON is a member of the MET family of receptor tyrosine kinases. When activated by its ligand, macrophage stimulating protein (MSP), RON initiates downstream signaling events that result in cell dissociation, migration, and invasion. Several RON variants that arise through mRNA splicing or by an alternate translational start site have been identified, some with oncogenic potential. The purpose of the present study was to characterize and determine any pathogenic roles of a naturally occurring 52 kD N-terminal truncated RON (sf-RON). Sf-RON was found to be overexpressed in 37% of 35 primary and 30% of 10 metastatic colorectal carcinomas (CRCs). Sf-RON was also expressed and constitutively phosphorylated in three CRC cell lines. Cloning sf-RON from a CRC cell line and transfecting it into AA/C1 colon epithelial cells resulted in constitutive activation with increased Map kinase p44/42 (ERK1/2) phosphorylation. Sf-RON expression also caused a decrease in E-cadherin, an important cell adhesion molecule whose downregulation is associated with poor survival in cancer. These changes in cell signaling were accompanied by a change in cell-shape and increased cellular growth and migration. Taken together, sf-RON may play a role in the progression of CRC to a more malignant phenotype.