Browsing by Subject "Callus (Botany)"
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Item Callus induction, maintenance and regeneration of interspecific allium hybrids(Texas Tech University, 1987-08) Lu, Chung-chengA culture system for plant regeneration via somatic embryogenesis of interspecific F1 hybrids was developed. Inflorescence and basal plate of 25 genotypes of interspecific Allium fistulosum x ^. cepa F. hybrids were cultured. Basal BDS medium containing 0.75 mg/1 picloram and 2.0 mg/a BA was ideal to induce and maintain callus culture. Callus regeneration was enhanced by proline and vitamins. Embryogenesis was regenerated on 2 media from 6 out of 25 genotypes of basal plate explant. Inflorescence explant contained higher regeneration potential and regenerated from 15 out of 16 treatments. Somaclonal variants were obtained. No karyotypic analyses were conducted so no putative chromosomal abnormalities occurring during culture were observed. Furthermore, variable isozyme banding patterns were found in Adh and Pgi.Item Development of cotton callus initiation medium and in vitro screening for osmotic stress tolerance(Texas Tech University, 1987-05) Zimmerman, Thomas WAsceptic excised seed hypocotyls of the Gossypium hirsutum L. varieties Acala 1517-75, Coker 500, Dunn 120, Paymaster 303, and TMl, and the exotic cotton lines, Tl, T25, and T133, were cultured for 24 days on six media and two gelling agents, Difco bacto agar and Kelco Gelrite. Significantly more callus was initiated on media solidified with Gelrite than with agar. The Linsmaier and Skoog medium (21) supplimented with 30 g/1 glucose, 0.1 mg/1 BA, and 0.1 mg/1 2,4-D supported significantly more callus production than the other five media. Exotic cottons, representing the seven races, were subjected to polyethylene glycol (PEG) induced osmotic stress in vitro in modified Linsmaier and Skoog (21) medium. The PEG levels were 0, 10, 20, and 30% w/v representing osmotic stresses of -5, -10, -16, and -24 bars respectively- The rate of dry matter accumulation, measured as dry cell weight (DCW), varied among cotton lines at each PEG level. The differences in density, DCW divided by packed cell volume, among the cotton cell lines at the stress levels indicate osmotic adjustment by the cells.