Browsing by Subject "Affinity chromatography"
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Item Applications of affinity chromatography to isolation of sequence specific nuclear proteins(Texas Tech University, 1981-05) Sherrod, Peter D.Even before Watson and Crick proposed their brilliant model for the structure of DNA, Jacob and Monod were busy piecing together the information that would lead to the first fully defined mechanism for genetic regulation in the bacteria, Escherichia coli. Since then, other mechanisms have become fell defined in prokaryotes, however eukaryotic mechanisms of control have been difficult to establish. This is primarily due to the fact that the genome of euiiaryotic cells is much more complex than in bacteria. Just the immensity of size defies the precise and controlled study that has yielded so much information in prokaryotic systems. Nevertheless, it is not inconceivable that similar mechanisms of regulation exist in the eukaryotic genome.Item Enhanced Purification of Platelet Beta-Lysin(Texas Tech University, 1982-05) Rickaby, Julia MarieExperimental alterations in the purification scheme established by Donaldson et al. for platelet B-lysin from rabbit serum were made with the objectives of enhanced bactericidal activity and increased stability. Either bovine serum albumin or reduced glutathione, when added to the filtrates in the purification scheme, increased bactericidal activity against Bacillus subtilis and Escherichia coli. In addition, ammonium bicarbonate was a more effective eluant than sodium chloride, which was used in the established procedure for removing 6-lysin from asbestos filter pads. The modified procedure, using glutathione and ammonium bicarbonate, yielded a stable 6-lysin preparation with increased bactericidal activity. Three UV absorbance peaks were obtained after filtration on a Sephadex G-50 column, with the third and largest peak showing substantial bactericidal activity. Bactericidal activity was also demonstrated in g-lysin samples after affinity chromatography on Affi-Gel blue. Analysis of the various 6-lysin preparations by polyacrylamide gel electrophoresis showed that the affinity chromatography step reduced albumin contamination in the samples of platelet 6-lysin.Item Item Synthesis of caryolanemagnolol and clovanemagnolol derivatives for molecular pull-down experiments(2011-12) Mitra, Aurpon W.; Siegel, Dionicio R.; Dong, GuangbinCaryolanemagnolol and clovanemagnolol promote neuronal regeneration in various cell and animal based assays. The protein targets of these natural products are not currently known. Derivatives of caryolanemagnolol and clovanemagnolol were synthesized for the purpose of affinity chromatography. The derivatives are accessed rapidly through optimized procedures.Item The interaction of ferredoxin with chloroplast ferredoxin-linked enzymes(Texas Tech University, 1987-08) Boyer, James MiltonNOT AVAILABLE