Browsing by Author "Banerjee, Nivedita"
Now showing 1 - 2 of 2
Results Per Page
Sort Options
Item Polyphenol-induced Anti-inflammatory and Cytotoxic Activities in Breast and Colon Cancer: Potential Role of miRNA's in Cell Survival and Inflammation(2013-12-11) Banerjee, NiveditaCancer is a major cause of death worldwide. Hence, there is a great need to develop novel therapeutic agents for the prevention and treatment of cancer in addition to conventional therapies. Dietary polyphenols are known to be effective in the prevention and treatment of several chronic diseases such as cancer, cardiovascular diseases, and diabetes. Particularly in carcinogenesis, polyphenols are known to suppress cancer growth, angiogenesis, and metastasis. Several studies have demonstrated that polyphenolics, ellagitannins, gallotannins, and chlorogenic acid from pomegranate, mango, and plum juice, respectively, are potent inhibitors of cancer cell proliferation and induce apoptosis and cell cycle arrest as well as decrease inflammation in vitro and vivo. The therapeutically relevant compounds in pomegranate are pelagic acid, ellagitannins, flavonoids, and 3-glucosides/3,5-diglucosides of the anthocyanins delphinidin, cyanidin, and pelargonidin that exerted antioxidant, anti-inflammatory, and anticarcinogenic activities in vitro and vivo. Mango pulp extract contains gallotannins, gallic acid, galloyl glycosides, and flavonoids such as quercetin and kaempferol glycosides, which showed antioxidant, anti-inflammatory, and anticarcinogenic activities in vitro and in vivo. Chlorogenic acid and neo-chlorogenic acid are contained in plum juice and are also known to function as chemoprevention and chemotherapeutic agents. The overall objective of this work was to investigate the underlying anti- inflammatory and cytotoxic mechanisms involving miR-27a-ZBTB10-Sp and miR-155- SHIP-1-PI3K axes, miR126-VCAM-1, miR126-PI3K/AKT-mTOR and miR143/PI3K/AKT/mTOR axes in polyphenol-mediated anti-inflammatory and anticarcinogenic activities in vitro and vivo. Pomegranate and Mango polyphenols exhibited antioxidant, anti-inflammatory, anticarcinogenic, and antiproliferative activities in vitro and in vivo. Polyphenols inhibited cell proliferation of breast cancer cell line BT474 and suppressed tumor growth in athymic BALB/c nude mice with BT474 xenografts. Interactions of Pg with miR-27a- ZBTB10-Sp and miR-155-SHIP-1-PI3K axes and mango miR126/PI3K/AKT axis were identified. In addition, pomegranate and plum polyphenols exerted cytotoxic and anti- inflammatory effects in azoxymethane AOM-treated rats and colon cancer cells. Interactions of Pg with miR126/VCAM-1 and miR126/PI3K/AKT/mTOR axes and plum with miR143/PI3K/AKT/mTOR were identified as mechanisms that at least in part appear to be involved in the anti-inflammatory and antiproliferative activities of pomegranate and plum polyphenolics. The presented research was conducted in order to understand the efficacy of polyphenols present in pomegranate, mango and plum and their underlying molecular mechanisms in different cancer models.Item Systematic Approach to Compare the Inflammatory Response of Liver Cell Culture Systems Exposed to Silver, Copper, and Nickel Nanoparticles(2011-10-21) Banerjee, NiveditaAlthough nano-sized metal colloids are used in industrial and medicinal applications, little is known about the potential liver toxicity of these materials after occupational or intentional exposures. To begin to resolve some outstanding hepatotoxicity concerns, the inflammatory response of hepatocytes after exposure to metal colloids was assessed. Four ~30-nm-sized metal colloids, including silver (nano-Ag), copper (nano-Cu) and nickel (nano-Ni) were examined in an effort to understand the induced cytokine expression in a murine liver cell line (AML12). Here we also utilized another system, co-cultures of hepatocytes, Kupffer?s cells, and lymphocytes isolated from C57BL6 mice. Cells were exposed to the materials over dose-response (0.1mg/L to 1000mg/L) and time-dependent (4 h, 48 h, and 1-week) studies. Cytotoxicity was measured via metabolism of resazurin and validated via MTT assay and cell counts. Inflammatory response was determined by cytokine profiles (TNF-a and IL-6), as well as by mRNA and protein expression of heat shock protein (Hsp70). Results from cells exposed to nano-Ag to doses of up to 100mg/L exhibited no significant changes in cytotoxicity, IL-6, or TNF-a production, or Hsp70 expression. Both nano-Cu and nano-Ni exposed cells exhibited decreased metabolism, increased Hsp70 induction, and increased inflammatory responses (IL-6 and TNF-a). Dynamic light scattering and electron microscopy were used to characterize particle size and surface charge. All three metal colloidal systems demonstrated different particle size distributions, agglomerated sizes, and surface zeta potentials. Furthermore, each metal colloid system elicited different inflammatory biomarker responses and stress protein expression.