Keatinge-Clay, Adrian Tristan2013-10-082017-05-112017-05-112013-05May 2013http://hdl.handle.net/2152/21485textPolyketide products are useful compounds to research and industry but can be difficult to access due to their richness in stereogenic centers. Type I polyketide synthases offer unique engineering opportunities to access natural stereocontrol and resultant complex compounds. The development of a controlled in vitro platform based around type I polyketide synthases is described. It has been used to produce a small library of polyketide fragments on an unprecedented and synthetically-relevant scale and explore polyketide synthase enzymology.application/pdfen-USIn vitroCell-freePreparativePolyketidePolyketide synthaseKetoreductaseMalonyl-CoA ligaseMatBEnzymologyChiral building blocksChiral synthonsDiketideTriketideLactoneKetolactoneNatural productsSecondary metabolismSecondary metaboliteModularType I polyketide synthaseBiosynthesisStreptomyces coelicolorSaccharopolyspora erythraeaBacillus subtilisStreptomyces nodosusSaccharomyces cerevisiaeStreptomyces antibioticusStreptomyces venezuelaeSaccharopolyspora spinosaStreptomyces fradiae2013-10-08