Path of DNA within Mu transpososomes: order, dynamics and topology of Mu end-enhancer interactions

dc.contributor.advisorJayaram, Makkunien
dc.contributor.advisorHarshey, Rasika M.en
dc.creatorPathania, Shailjaen
dc.date.accessioned2008-08-28T21:36:37Zen
dc.date.available2008-08-28T21:36:37Zen
dc.date.issued2002en
dc.descriptiontexten
dc.description.abstractUsing a new method called ‘difference topology’, a complete topological analysis of all the intermediates in the Mu transposition pathway has been carried out. The results give us a first look into the intricate architecture of transpososomes made by the Mu transposase. We show that the three participating DNA sites - L (left end), R (right end) and E (enhancer) - are brought together to make an elaborate 5-noded synapse, wherein E crosses R twice and L once, and L and R cross each other two times. This is the most complex DNA arrangement seen to date within a recombination synapse. The order and dynamics of association of these sites was monitored within a series of transpososomes prior to and during synapsis (LER), engagement (type 0), cleavage (type I) and strand transfer (type II) of Mu ends. In the process, we have discovered a new intermediate ER, which likely initiates the assembly pathway. We have found that the enhancer, previously believed to have exited the transpososome as early as in the type 0 complex, instead remains within the transpososome through completion of transposition. Our results suggest that the enhancer may play additional roles in transposition than previously thought.
dc.description.departmentMicrobiologyen
dc.format.mediumelectronicen
dc.identifierb5717698xen
dc.identifier.oclc56840688en
dc.identifier.proqst3110672en
dc.identifier.urihttp://hdl.handle.net/2152/840en
dc.language.isoengen
dc.rightsCopyright is held by the author. Presentation of this material on the Libraries' web site by University Libraries, The University of Texas at Austin was made possible under a limited license grant from the author who has retained all copyrights in the works.en
dc.subject.lcshDNA--Structureen
dc.subject.lcshBacteriophage muen
dc.titlePath of DNA within Mu transpososomes: order, dynamics and topology of Mu end-enhancer interactionsen
dc.type.genreThesisen

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