Overexpression of higher plant nitrite reductase

dc.creatorLi, Xin
dc.date.accessioned2016-11-14T23:10:39Z
dc.date.available2011-02-19T00:32:14Z
dc.date.available2016-11-14T23:10:39Z
dc.date.issued1999-05
dc.degree.departmentChemistryen_US
dc.description.abstractThe gene encoding the ferredoxin-dependent nitrite reductase of spinach chloroplasts (NiR; EC 1.7.7.1) has been isolated and sequenced (Back et ah, 1988). Although some information is available about the possible role of a few specific amino acids in the enzyme mechanism (Hirasawa et ah, 1994; Bellissimo et fl/.,1995; Dose et ah, 1997 and Dose, 1996), the absence of a 3-dimensional structure for the protein and the scarcity of data obtained with site-specific mutants have left considerable uncertainty about the enzyme mechanism. The work presented in this dissertation represents an attempt to address, in part, these issues by designing a system to over-express NiR in Escherichia coli and investigate the roles of the tryptophan residue at position 92 in the enzyme mechanism.
dc.format.mimetypeapplication/pdf
dc.identifier.urihttp://hdl.handle.net/2346/21752en_US
dc.language.isoeng
dc.publisherTexas Tech Universityen_US
dc.rights.availabilityUnrestricted.
dc.subjectChloroplastsen_US
dc.subjectRecombinant DNAen_US
dc.subjectNitrogen compounds -- Synthesisen_US
dc.subjectEnzymes -- Metabolismen_US
dc.subjectEnzymes -- Synthesisen_US
dc.titleOverexpression of higher plant nitrite reductase
dc.typeDissertation

Files