High-throughput analysis of emerging and historical pollutants in biological matrices.

dc.contributor.advisorUsenko, Sascha.
dc.contributor.authorSubedi, Bikram.
dc.contributor.departmentChemistry and Biochemistry.en_US
dc.contributor.schoolsBaylor University. Dept. of Chemistry and Biochemistry.en_US
dc.date.accessioned2013-05-15T18:53:55Z
dc.date.accessioned2017-04-07T19:34:39Z
dc.date.available2013-05-15T18:53:55Z
dc.date.available2017-04-07T19:34:39Z
dc.date.copyright2012-12
dc.date.issued2013-05-15
dc.description.abstractThe Deepwater Horizon Gulf Spill highlighted key deficiencies associated with our ability to analyze contaminants in biological tissue in a timely manner. These deficiencies in laboratory capacity and preparedness demonstrated a critical need to develop and validate high-throughput analytical methods capable of rapid and accurate quantification of organic pollutants in biological tissue from aquatic environments. High-throughput methods offer distinct advantages over historical methods, namely a reduction in the intrinsic costs associated with the sample preparation: time, solvents, labor, laboratory space, training, and potential loss of analytes. Sample preparation steps for the analysis of organic pollutants represent ~2/3 of the analytical cost, and are often the logistical bottleneck for laboratory capacity and preparedness. An enhanced pressurized liquid extraction (ePLE) method was developed for simultaneous analysis of pharmaceuticals and personal care products (PPCPs) in fish tissue and was utilized in a nationwide PPCP study conducted in Germany. The developed high-throughput method incorporated silica gel cleanup with PLE and improved the method sensitivity by optimizing resonant collision induced dissociation energy through ion-trap mass analyzer. The nationwide PPCP study found that the proximity of sampling locations to the upstream wastewater treatment plant discharging point and mean annual flow at the sampling locations were found to significantly powerful predictor of galaxolide and tonalide fish tissue concentrations (galaxolide: r2 = 0.79, p = 0.021 and tonalide: r2 = 0.81, p = 0.037) in Germany. Galaxolide and tonalide, both polycyclic musk fragrance materials, in German fish tissues were ~19 and ~28× lower, respectively, as compared to similar nationwide PPCP study in the United States. Similarly, a high-throughput ePLE method was developed for polychlorodibenzo-p-dioxins/furans (PCDD/Fs) and dioxin-like polychlorobiphenyls (dl-PCBs) by incorporating pressurized liquid extraction and multiple cleanup techniques. Sample preparation time and solvents were reduced as much as 95% and 65%, respectively, as compared to a conventional USEPA Method 1613. ePLE extracts were sufficiently clean for high resolution mass spectrometry analysis of PCDD/Fs and dl-PCBs in fish tissues from San Jacinto River Waste Pits, a Superfund site in Houston, TX.en_US
dc.description.degreePh.D.en_US
dc.identifier.citationSubedi, B., Mottaleb, M. A., Chambliss, C. K., Usenko, S. "Simultaneous analysis of select pharmaceuticals and personal care products in fish tissue using pressurized liquid extraction combined with silica gel cleanup." J. Chromatogr. A 1218, 37 (2011): 6278-6284.en_US
dc.identifier.citationSubedi, B., Du, B., Chambliss, C. K., Koschorreck, J., Rüdel, H., Quack, M., Brooks, B. W., Usenko, S. "Occurrence of pharmaceuticals and personal care products in German fish tissue : a national study." Environ. Sci. Technol. 46, 16 (2012): 9047-9054.en_US
dc.identifier.citationSubedi, B., Usenko, S. "Enhanced pressurized liquid extraction technique capable of analyzing polychlorodibenzo-p-dioxins, polychlorodibenzofurans, and polychlorobiphenyls in fish tissue." J. Chromatogr. A 1238 (2012): 30-37.en_US
dc.identifier.urihttp://hdl.handle.net/2104/8597
dc.language.isoen_USen_US
dc.publisheren
dc.rightsBaylor University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. Contact librarywebmaster@baylor.edu for inquiries about permission.en_US
dc.rights.accessrightsWorldwide access.en_US
dc.rights.accessrightsAccess changed 5/21/14.
dc.subjectEnhanced pressurized liquid extraction technique.en_US
dc.subjectHigh-throughout analytical techniques.en_US
dc.subjectGerman environment specimen bank samples.en_US
dc.subjectHouston shipping channel, a superfund site.en_US
dc.subjectChlorinated dioxins, furans, and biphenyls.en_US
dc.subjectFate and transport of organic contaminant.en_US
dc.subjectPharmaceuticals and personal care products.en_US
dc.titleHigh-throughput analysis of emerging and historical pollutants in biological matrices.en_US
dc.typeThesisen_US

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