The focus of this study is to investigate the effect of semen thaw procedures following a timed AI protocol in first calf females. British cross (Angus, Hereford) cows (n = 241) were synchronized utilizing a 7-day Co-Synch protocol. Females were randomly divided into 2 breeding days and received either technician thawed semen or water regulated thawed semen. Pregnancy status × thaw method did not prove to be statistically significant (P = 0.51), neither did pregnancy status × AI technician (P = 0.97) or pregnancy status × AI day (P = 0.30). The probability of pregnancy for TT and WR was not different (P = 0.53).