Callus induction, maintenance and regeneration of interspecific allium hybrids
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A culture system for plant regeneration via somatic embryogenesis of interspecific F1 hybrids was developed. Inflorescence and basal plate of 25 genotypes of interspecific Allium fistulosum x ^. cepa F. hybrids were cultured. Basal BDS medium containing 0.75 mg/1 picloram and 2.0 mg/a BA was ideal to induce and maintain callus culture. Callus regeneration was enhanced by proline and vitamins. Embryogenesis was regenerated on 2 media from 6 out of 25 genotypes of basal plate explant. Inflorescence explant contained higher regeneration potential and regenerated from 15 out of 16 treatments. Somaclonal variants were obtained. No karyotypic analyses were conducted so no putative chromosomal abnormalities occurring during culture were observed. Furthermore, variable isozyme banding patterns were found in Adh and Pgi.