The Investigation of the Effects of Low Light Laser Therapy on Insulin Secretion in Porcine Islets, a Pilot Study
Raptis, Darren James
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LED light technology is used in this pilot study to demonstrate the ability to create a cost effective light apparatus using 1-watt LEDs with wavelengths of 740nm,850nm, and 940nm to test for an increased insulin response using porcine islet of Langerhans cells. This is building on recent research showing increased insulin response in rat islet cells when exposed to LED light. However, due to the high variance and low sample size, statistically significant changes were not measured when irradiating cells with 740nm, 850nm, and 940nm LED light using 7.9 J/cm2 and 15.8 J/cm2 dosages along with low and high glucose conditions. There are however possible trends of increased insulin secretion that may become significant with increased sample size. There is a need to repeat this study to definitively determine if increased insulin secretion does occur in porcine islet of Langerhans cells along with any potential negative effects and what the optimal wavelengths and dosages would be. Using the cost effective light apparatus, additional wavelengths can be experimented with to cover between 300nm to over 1600nms. The information garnered from this research has the potential to improve islet of Langerhans cell transplantation for type one diabetes (T1D) patients by decreasing the number of cells needed to be therapeutic and increasing insulin release.